Full Product Name
GCK Antibody - middle region
Product Gene Name
anti-GCK antibody
[Similar Products]
Product Synonym Gene Name
GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3[Similar Products]
Antibody/Peptide Pairs
GCK peptide (MBS3248549) is used for blocking the activity of GCK antibody (MBS3224221)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: LLFHGEASEQ LRTRGAFETR FVSQVESDTG DRKQIYNILS TLGLRPSTTD
3D Structure
ModBase 3D Structure for P35557
Purity/Purification
Affinity purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human GCK
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-GCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GCK antibody
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Product Categories/Family for anti-GCK antibody
Polyclonal; Membrane Protein; Mitochondria; Disease Related; Tissue Specific & Cell Marker;
Applications Tested/Suitable for anti-GCK antibody
Western Blot (WB)
Western Blot (WB) of anti-GCK antibody
Host: Rabbit
Target Name: GCK
Sample Tissue: Human LN18 Whole Cell lysates
Antibody Dilution: 1ug/ml
NCBI/Uniprot data below describe general gene information for GCK. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000153.1
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NCBI GenBank Nucleotide #
NM_000162.3
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UniProt Primary Accession #
P35557
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UniProt Related Accession #
P35557[Other Products]
NCBI Official Full Name
glucokinase isoform 1
NCBI Official Synonym Full Names
glucokinase
NCBI Official Symbol
GCK??[Similar Products]
NCBI Official Synonym Symbols
GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
??[Similar Products]
NCBI Protein Information
glucokinase
UniProt Protein Name
Glucokinase
UniProt Synonym Protein Names
Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
Protein Family
Glycerate 2-kinase
UniProt Gene Name
GCK??[Similar Products]
UniProt Synonym Gene Names
HK IV; HK4??[Similar Products]
UniProt Entry Name
HXK4_HUMAN
NCBI Summary for GCK
This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017]
Research Articles on GCK
1. Our results suggest that the polymorphism of CYP3A4 *18B and GCK G-30A might affect the new-onset diabetes after transplantation (NODAT) development under tacrolimus-based immunotherapy. We suspect that the depression of GCK function may be a crucial pathogenic factor of tacrolimus-induced NODAT in variant carrier with A allele of GCK, but not be simply reflecting of glucose change.
Precautions
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Disclaimer
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