Monoclonal GCK; Anti-GCK; MAP4K2; Mitogen-Activated Protein Kinase 2; BL44; RAB8IP

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

3 E3

Mouse

GCK antibody was purified by affinity chromatography.

Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.

500 ug-1 mg/ml (lot specific)

Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Small volumes of anti-GCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Mouse monoclonal GCK antibody

Proteases, Inhibitors, & Enzymes

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

FC: 1:500
IF: 1:100
IHC: 1:50
WB: 1:500

Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GCK protein (Right) or empty vector (Left) detected with GCK antibody

Immunohistochemical analysis of GCK protein in paraffin embedded Adenocarcinoma of Human breast tissue using GCK antibody

52,035 Da

glucokinase (hexokinase 4)

glucokinase

Glucokinase

HK IV; HK4??[Similar Products]

HXK4_HUMAN

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]

GK: a glycolytic enzyme that catalyzes glucose metabolism in the liver and pancreatic beta cells. Acts as a ""glucose sensor"" in beta cells. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. Glucokinase traps glucose inside the cell by catalyzing its phosphorylation to produce glucose-6-phosphate. Has a lower affinity for glucose than the three other isozymes of hexokinase, allowing other organs such as the brain and muscles to have first call on glucose when its supply is limited. Unlike other hexokinases, glucokinase is not inhibited by glucose-6-phosphate. Glucokinase is found in the outer membrane compartment of mitochondria. May bind VDAC, suppressing mitochondrial function. Glucokinase transcription is induced by insulin, perhaps via the activation of Stat 5B. Mutant glucokinase causes a rare form of diabetes and may also play a role in type 2 diabetes. Three splice variant isoforms of human glucokinase have been described.

Protein type: Mitochondrial; EC 2.7.1.2; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Kinase, other

Chromosomal Location of Human Ortholog: 7p15.3-p15.1

Cellular Component: nucleoplasm; mitochondrion; actin filament; cytosol; basal cortex; secretory granule

Molecular Function: protein binding; mannokinase activity; fructokinase activity; magnesium ion binding; ADP binding; glucokinase activity; protein phosphatase binding; ATP binding; glucose binding

Biological Process: glucose 6-phosphate metabolic process; positive regulation of glycogen biosynthetic process; lipid homeostasis; pathogenesis; glucose transport; glucose homeostasis; NADP metabolic process; cellular response to glucose starvation; elevation of cytosolic calcium ion concentration; detection of glucose; regulation of glycolysis; negative regulation of gluconeogenesis; transmembrane transport; second-messenger-mediated signaling; glycogen biosynthetic process; glycolysis; regulation of potassium ion transport; positive regulation of insulin secretion; glucose metabolic process; endocrine pancreas development; fructose 2,6-bisphosphate metabolic process; cellular response to insulin stimulus; hexose transport; carbohydrate metabolic process; positive regulation of glycolysis; carbohydrate phosphorylation; negative regulation of epinephrine secretion; cell glucose homeostasis; regulation of insulin secretion; positive regulation of phosphorylation

Disease: Hyperinsulinemic Hypoglycemia, Familial, 3; Diabetes Mellitus, Permanent Neonatal; Maturity-onset Diabetes Of The Young, Type 2; Diabetes Mellitus, Noninsulin-dependent

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