Rat Serotonin Receptor 2A/5-hydroxytryptamine Receptor 2A (5-HT-2A) ELISA Kit; RP11-147L20.2; 5-HT2A; HTR2; 5-HT2 receptor; 5-hydroxytryptamine receptor 2A; serotonin 5-HT-2A receptor; 5-hydroxytryptamine (serotonin) receptor 2A

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of rat 5-HT-2A. No significant cross-reactivity or interference between rat 5-HT-2A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of HTR2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS705328 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTR2A. The ELISA analytical biochemical technique of the MBS705328 kit is based on HTR2A antibody-HTR2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTR2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTR2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 5-HT-2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 5-HT-2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-HT-2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-HT-2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

52,850 Da

5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled

5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; serotonin receptor 2A; serotonin 5HT-2 receptor

5-hydroxytryptamine receptor 2A

Htr2; 5-HT-2; 5-HT-2A??[Similar Products]

5HT2A_RAT

a receptor for 5-hydroxytryptamine (serotonin); activates phospholipase C signaling pathway; involved in promoting cellular transformation [RGD, Feb 2006]

Function: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.2 PublicationsManual assertion based on experiment in:Ref.1

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