For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat HTR1A. No significant cross-reactivity or interference between Rat HTR1A and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of HTR1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282368 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine receptor 1A (HTR1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTR1A. The ELISA analytical biochemical technique of the MBS282368 kit is based on HTR1A antibody-HTR1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTR1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTR1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HTR1A in samples. An antibody specific for HTR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHTR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

46,429 Da

5-hydroxytryptamine receptor 1A

5-hydroxytryptamine receptor 1A

5-hydroxytryptamine receptor 1A

5ht1a; 5-HT-1A; 5-HT1A??[Similar Products]

regulates AMPA receptor channels in the prefrontal cortex; regulation is mediated via inhibition of Ca2+/calmodulin-dependent kinase II [RGD, Feb 2006]

5-HT(1A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. 5- hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: axon hillock; cell soma; integral component of plasma membrane; membrane

Molecular Function: drug binding; G-protein alpha-subunit binding; G-protein coupled serotonin receptor activity; neurotransmitter receptor activity; protein binding; receptor binding; serotonin binding

Biological Process: adenylate cyclase-inhibiting serotonin receptor signaling pathway; behavioral fear response; cell proliferation; G-protein coupled receptor signaling pathway; positive regulation of microtubule depolymerization; regulation of behavior; regulation of dopamine metabolic process; regulation of hormone secretion; regulation of serotonin secretion; serotonin metabolic process; serotonin receptor signaling pathway; vasoconstriction

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