Endoplasmic reticulum aminopeptidase 1; 3411-; ARTS-1; Adipocyte-derived leucine aminopeptidase; A-LAP; Aminopeptidase PILS; Puromycin-insensitive leucyl-specific aminopeptidase; PILS-AP; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; ERAP1; APPILS; ARTS1; KIAA0525

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Purified Rabbit Polyclonal Antibody (Pab)

0.01M PBS, pH 7.2, 0.1% Sodium azide, Glycerol 50%

1mg/ml (lot specific)

Store at -20 degree C.Stable for 12 months from date of receipt

Small volumes of anti-ERAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.

Cardiovascular; Cell Biology; Metabolism; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC)-Paraffin

WB~~ 1:1000

Anti-ERAP1 Antibodyat 1:1000 dilution + K562 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution

Predicted band size : 107 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

107235

endoplasmic reticulum aminopeptidase 1

endoplasmic reticulum aminopeptidase 1

Endoplasmic reticulum aminopeptidase 1

APPILS; ARTS1; KIAA0525; A-LAP; PILS-AP??[Similar Products]

ERAP1_HUMAN

The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]

ARTS1: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. Belongs to the peptidase M1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.11.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; endoplasmic reticulum lumen; integral to membrane; extracellular region; cytosol

Molecular Function: protein binding; interleukin-6 receptor binding; metalloexopeptidase activity; zinc ion binding; interleukin-1, Type II receptor binding; aminopeptidase activity

Biological Process: fat cell differentiation; positive regulation of angiogenesis; antigen processing and presentation of peptide antigen via MHC class I; response to bacterium; regulation of blood pressure; membrane protein ectodomain proteolysis; angiogenesis; antigen processing and presentation of endogenous peptide antigen via MHC class I; regulation of innate immune response

Hattori A.,et al.J. Biochem. 125:931-938(1999).
Hattori A.,et al.J. Biochem. 130:235-241(2001).
Schomburg L.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Cui X.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Nagase T.,et al.DNA Res. 5:31-39(1998).

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