For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

Mouse

This antibody recognizes human and rat LRRK2.

100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

This product is stable for at least one (1) year at-20 degree C.

Small volumes of anti-LRRK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Leucine-rich repeat kinase 2 (LRRK2), also known as dardarin, is an enzyme that in humans is encoded by the PARK8 gene. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson's disease type 8 and Crohn's disease which suggests that these two diseases share a common pathway. Expression of mutant LRRK2 induced apoptotic cell death in neuroblastoma cells and in mouse cortical neurons.

Immunoblot, Immunofluorescence (IF)

Immunoblot: 1-5ug/ml. A band of >200kDa is detected
IF: 10ug/ml
Dilute in PBS or medium that is identical to that used in the assay system.

Detection of LRRK2 in rat brain lysate at 5ug/ml.

Detection of LRRK2 in neuroblastoma cell line SK-N-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) LRRK2 stain.

286,103 Da

leucine rich repeat kinase 2

leucine-rich repeat serine/threonine-protein kinase 2

Leucine-rich repeat serine/threonine-protein kinase 2

PARK8??[Similar Products]

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]

Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. Regulates neuronal process morphology in the intact central nervous system (CNS). Plays a role in synaptic vesicle trafficking. Phosphorylates PRDX3. Has GTPase activity. May play a role in the phosphorylation of proteins central to Parkinson disease.

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