ERAP1 Antibody : ALAP; A-LAP; ARTS1; ERAAP; APPILS; ARTS-1; ERAAP1; PILSAP; PILS-AP; KIAA0525; UNQ584/PRO1154; Endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase 1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

ERAP1 antibody is human and mouse reactive. At least two isoforms of ERAP1 are known to exist; this antibody will detect both isoforms. ERAP1 antibody is predicted to not cross-react with ERAP2.

ERAP1 Antibody is affinity chromatography purified via peptide column.

Liquid

1 mg/mL (lot specific)

ERAP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Small volumes of anti-ERAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1), a member of the peptidase M1 family, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1,2). It is also designated as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1) (3). ERAP1 is localized to the lumen of the ER and induced by interferon. It may be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (3,4).

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF)

ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 mug/ml.

Optimal dilutions for each application to be determined by the researcher.

Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 μg/ml.

Immunohistochemistry of ERAP1 in mouse brain tissue with ERAP1 antibody at 5 μg/mL.

Immunofluorescence of ERAP1 in mouse brain tissue with ERAP1 antibody at 20 μg/mL.

Predicted: 104 kDa

Observed: 105kDa

endoplasmic reticulum aminopeptidase 1

endoplasmic reticulum aminopeptidase 1; aminopeptidase PILS; adipocyte-derived leucine aminopeptidase; aminopeptidase regulator of TNFR1 shedding; puromycin-insensitive leucyl-specific aminopeptidase; endoplasmic reticulum aminopeptidase 1 delta-Exon-11 isoform; endoplasmic reticulum aminopeptidase 1 delta-Exon-13 isoform; endoplasmic reticulum aminopeptidase 1 delta-Exon-14 isoform; endoplasmic reticulum aminopeptidase 1 delta-Exon-15 isoform; endoplasmic reticulum aminopeptidase associated with antigen processing; type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

Endoplasmic reticulum aminopeptidase 1

APPILS; ARTS1; KIAA0525; A-LAP; PILS-AP??[Similar Products]

ERAP1_HUMAN

The protein encoded by this gene is an aminopeptidase involved in trimming HLA class I-binding precursors so that they can be presented on MHC class I molecules. The encoded protein acts as a monomer or as a heterodimer with ERAP2. This protein may also be involved in blood pressure regulation by inactivation of angiotensin II. Three transcript variants encoding two different isoforms have been found for this gene.[provided by RefSeq, Oct 2010]

ARTS1: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I- binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney. Belongs to the peptidase M1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.11.-; Protease

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: extracellular space; endoplasmic reticulum membrane; membrane; endoplasmic reticulum lumen; endoplasmic reticulum; integral to membrane; extracellular region; cytosol

Molecular Function: protein binding; interleukin-6 receptor binding; metalloexopeptidase activity; zinc ion binding; interleukin-1, Type II receptor binding; aminopeptidase activity

Biological Process: fat cell differentiation; positive regulation of angiogenesis; antigen processing and presentation of peptide antigen via MHC class I; response to bacterium; regulation of blood pressure; membrane protein ectodomain proteolysis; angiogenesis; antigen processing and presentation of endogenous peptide antigen via MHC class I; regulation of innate immune response

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