Anti-ABCB5 Antibody (N-Terminus) IHC-plus; ABCB5; ABCB5alpha; ABCB5 P-gp; ABCB5beta; ATP-binding cassette protein; EST422562; P-glycoprotein ABCB5; Human ABCB5

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Ammonium sulfate precipitation

PBS, 0.09% sodium azide

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Small volumes of anti-ABCB5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Subfamily:-ATP-binding-cassette---ABCB/MDR">Family: Transporter
Subfamily: ATP-binding cassette - ABCB/MDR

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)

Flo (1:10 - 1:50), IHC-P (10 ug/ml), WB (1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Anti-ABCB5 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver

Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver

Western blot analysis of ABCB5 Antibody (N-term) in A375, K562, A2058 and HL-60 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified Pab.

Western blot of K562 cell line lysates (35 ug/lane).

89,832 Da

ATP-binding cassette, sub-family B (MDR/TAP), member 5

ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5; ATP-binding cassette protein

ATP-binding cassette sub-family B member 5

ABCB5_HUMAN

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter; Transporter, ABC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: regulation of membrane potential; metabolic process; compound eye corneal lens development; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; cell differentiation; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; transmembrane transport; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent

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