For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 12; NC_000012.11 (8754762..8765463, complement). Location: 12p13

Recognizes human Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2). Species Crossreactivity: mouse.

Highly Purified
Highly Purified

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

Liquid peptide is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of AICDA enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Synthetic peptide corresponding to 14 amino acids near the carboxy-terminus of human AID (GenBank accession no. AAM95402). See corresponding antibody A0855-75J.

Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNAediting cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines (1). It is necessary for somatic hypermutation and class switch recombination in B cells (2), but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms (2,3). Although it is structurally and functionally similar to the APOBEC proteins (reviewed in 4), it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G (5).

Molecular Biology; MB-Enzymes

Suitable for use as a blocking peptide in ELISA, Western Blot with A0855-75J.
Dilution: Western Blot: AID peptide is used for blocking the activity of AID antibody (Cat. No. A0855-75J). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37 C.

23,954 Da[Similar Products]

activation-induced cytidine deaminase

single-stranded DNA cytosine deaminase; cytidine aminohydrolase; integrated into Burkitt's lymphoma cell line Ramos

Single-stranded DNA cytosine deaminase

AID??[Similar Products]

AICDA_HUMAN

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]

Function: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Ref.9 Ref.10 Ref.11 Ref.13

Catalytic activity: Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.

Cofactor: Zinc

By similarity.

Subunit structure: Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1. Interacts with TRIM28 and NCL

By similarity. Interacts with SUPT6H. Ref.8 Ref.9 Ref.11 Ref.13

Subcellular location: Nucleus. Cytoplasm. Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm. Ref.7 Ref.9 Ref.11 Ref.12

Tissue specificity: Strongly expressed in lymph nodes and tonsils.

Post-translational modification: Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity.

Involvement in disease: Immunodeficiency with hyper-IgM 2 (HIGM2) [MIM:605258]: A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2

Sequence similarities: Belongs to the cytidine and deoxycytidylate deaminase family.

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