Anti-TG / Thyroglobulin Antibody (clone 2H11) IHC-plus; TG; AITD3; TDH3; Thyroglobulin; TGN; Human TG; Thyroglobulin

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG1

2H11

Mouse

Human thyroglobulin protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.

Protein A Purified

0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.

1 mg/ml (lot specific)

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Small volumes of anti-TG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Thyroglobulin (TG) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen.

Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)

ELISA, IHC-Fr (1:50 - 1:200), IHC-P (5 ug/ml), IP, WB (1:400)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Anti-TG / Thyroglobulin antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-Thyroglobulin Antibody - Western Blot. Western blot of Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kD band corresponding to human thyroglobulin (left lane 3 ug, right lane 3.0 ug) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.

Western blot of anti-Thyroglobulin antibody at a dilution of 1:400. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 mg, right lane 3.0 mg) as confirmed by the position of molecular weight markers (not shown).

298,383 Da

thyroglobulin

thyroglobulin

Thyroglobulin

Tg??[Similar Products]

THYG_HUMAN

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.