Sheep matrix metallopeptidase 1 (interstitial collagenase) (MMP1) ELISA kit; CLG; CLGN; fibroblast collagenase; matrix metalloprotease 1; matrix metalloproteinase 1; matrix metallopeptidase 1 (interstitial collagenase) (MMP1)

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of sheep MMP1. No significant cross-reactivity or interference between sheep MMP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of interstitial collagenase elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS706095 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the matrix metallopeptidase 1 (interstitial collagenase) (MMP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing interstitial collagenase. The ELISA analytical biochemical technique of the MBS706095 kit is based on interstitial collagenase antibody-interstitial collagenase antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect interstitial collagenase antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, interstitial collagenase. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

46,691 Da

succinate-CoA ligase, GDP-forming, beta subunit

succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial; succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial; SCS-betaG; succinyl-CoA synthetase beta-G chain; succinate-CoA ligase, GDP-forming beta subunit; GTP-specific succinyl-CoA synthetase subunit beta

Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial

SCS-betaG??[Similar Products]

SUCB2_BOVIN

Function: Catalyzes the GTP-dependent ligation of succinate and CoA to form succinyl-CoA

Catalytic activity: GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.

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