Anti -HLA Class 1 Antigen B27 (HLA Class I Histocompatibility Antigen B-27 alpha Chain, HLA B27 Class I, HLA-B27, MHC Class I Antigen B*27)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 6; NC_000006.11 (31321649..31324989, complement). Location: 6p21.3

Monoclonal

IgM-K

EP-4

Mouse

Recognizes the HLA-B27 cell surface antigen on human cells.

Purified
Purified

Supplied as a liquid in PBS, 0.05% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-HLA-B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Since EP-4 recognizes the HLA-B27 antigen, it may be used to HLA type human lymphoctyes. Approximately 60% of patients with ankylosing spondylitis are HLA-B27 positive. This reagent can be used to help identify this HLA haplotype in human lymphocytes. Monoclonal antibody EP-4 recognizes the HLA-B27 antigen expressed on individuals with this HLA haplotype.

Antibodies; Abs to HLA

Western Blot (WB), Immunocytochemistry (ICC)

Suitable for use in Western Blot and Immunocytochemistry.
Dilution: Immunocytochemistry: Paraformaldehyde fixed cell suspensions or cytospins

40,460 Da[Similar Products]

major histocompatibility complex, class I, B

major histocompatibility complex, class I, B; lymphocyte antigen; MHC class I antigen SHCHA; MHC Class I HLA heavy chain; MHC class I antigen GN00104; leukocyte antigen class I-B; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen HLA-B heavy chain; HLA class I histocompatibility antigen, B alpha chain

HLA class I histocompatibility antigen, B-7 alpha chain

HLAB??[Similar Products]

1B07_HUMAN

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; endoplasmic reticulum; integral to plasma membrane; integral to membrane; plasma membrane; MHC class I protein complex

Molecular Function: peptide antigen binding; chaperone binding; TAP binding; receptor binding

Biological Process: regulation of immune response; regulation of interleukin-12 production; viral reproduction; detection of bacterium; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; protection from natural killer cell mediated cytotoxicity; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; regulation of T cell anergy; regulation of interleukin-6 production; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.