5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3; 5HT3 serotonin receptor|serotonin-gated ion channel receptor

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Guinea pig HTR3A. No significant cross-reactivity or interference between Guinea pig HTR3A and analogues was observed.

Store at 2-8 degree C.

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Small volumes of HTR3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS286023 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine receptor 3A (HTR3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTR3A. The ELISA analytical biochemical technique of the MBS286023 kit is based on HTR3A antibody-HTR3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTR3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTR3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HTR3A in samples. An antibody specific for HTR3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHTR3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTR3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTR3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

55,087 Da

5-hydroxytryptamine receptor 3A

5-hydroxytryptamine receptor 3A

5HT3R; HTR3; 5-HT3-A; 5-HT3A; 5-HT-3; 5-HT3R??[Similar Products]

5-HT(3): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel

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