Full Product Name
Anti-p63
Product Gene Name
anti-p63 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9H3D4
Buffer
20 mM Tris-HCl, pH 8.0
Immunogen
Peptide derived from the internal sequence of human p63. Antibody recognizes the epitope between Tyr231 - Arg251.
Cellular localization
nucleus
Positive control
tonsil tissue
IHC-P PROTOCOL
INSTRUCTION MANUAL
1. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer*, pH 9.0 and incubate at 95-97oC in water bath for 25 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 minutes.
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber.
READY TO USE (RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed3to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual is suggested
13. Wash 3 x 5 minutes with buffer A.
14. Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.
VENTANA PROTOCOL
INSTRUCTION MANUAL
SHORT APPLICATION PROTOCOL FOR VENTANA BENCHMARK SLIDE STAINING SYSTEM
1. Deparafinization (Enter).
2. Heating glass (72oC) with the medium temperatures. Deparafinization.
3. Prolonged deparafinization (Enter).
4. Cell conditioning (Enter).
5. ULTRA Conditioner #2 (Enter).
6. Heating glass (97°C), incubation 8 min (Cell conditioner #2).
7. ULTRA CC2 solution application ? 24 min (Enter).
8. Titration (Enter).
9. Hand apply ? primary antibody. Incubation 32 min.
10. UltraWash (Enter).
11. Nuclear stain (Enter).
12. Hematoxylin application ? one drop (nuclear stain). Cover and incubate 8 min.
13. After nuclear stain (Enter).
14. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min.
PRECAUTIONS
1. Intended for professional In Vitro Diagnostic use in laboratories.
2. Do not use after expiration date stamped on vial label.
3. Avoid contamination of the reagent.
4. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
5. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
6. Disposal of waste material must be conducted in accordance with local regulations.
7. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
Preparation and Storage
Storage: +4 degree C
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Other Notes
Small volumes of anti-p63 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-p63 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-p63 antibody
Immunohistochemistry (IHC) Paraffin: 1:100 - 1:200
Immunohistochemistry (IHC) of anti-p63 antibody
Immunohistochemistry (IHC) of anti-p63 antibody
NCBI/Uniprot data below describe general gene information for p63. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001108450.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001114978.1
[Other Products]
UniProt Primary Accession #
Q9H3D4
[Other Products]
UniProt Secondary Accession #
O75080; O75195; O75922; O76078; Q6VEG2; Q6VEG3; Q6VEG4; Q6VFJ1; Q6VFJ2; Q6VFJ3; Q6VH20[Other Products]
UniProt Related Accession #
Q9H3D4[Other Products]
Molecular Weight
65,288 Da
NCBI Official Full Name
tumor protein 63 isoform 2
NCBI Official Synonym Full Names
tumor protein p63
NCBI Official Symbol
TP63??[Similar Products]
NCBI Official Synonym Symbols
AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
??[Similar Products]
NCBI Protein Information
tumor protein 63
UniProt Protein Name
Tumor protein 63
UniProt Synonym Protein Names
Chronic ulcerative stomatitis protein; CUSP; Keratinocyte transcription factor KET; Transformation-related protein 63; TP63; Tumor protein p73-like; p73L; p40; p51
UniProt Gene Name
TP63??[Similar Products]
UniProt Synonym Gene Names
KET; P63; P73H; P73L; TP73L; p63; CUSP; TP63; p73L??[Similar Products]
UniProt Entry Name
P63_HUMAN
NCBI Summary for p63
This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. Many transcripts encoding different proteins have been reported but the
biological validity and the full-length nature of these variants have not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for p63
p63: Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L. Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Belongs to the p53 family. 12 isoforms of the human protein are produced by alternative promoter.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 3q28
Cellular Component: cytoplasm; cytosol; dendrite; nuclear chromatin; nucleoplasm; nucleus; rough endoplasmic reticulum; transcription factor complex
Molecular Function: chromatin binding; damaged DNA binding; DNA binding; double-stranded DNA binding; identical protein binding; metal ion binding; p53 binding; protein binding; sequence-specific DNA binding; transcription factor activity; WW domain binding
Biological Process: apoptosis; chromatin remodeling; cloacal septation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; ectoderm and mesoderm interaction; embryonic limb morphogenesis; epidermal cell division; epithelial cell development; establishment of planar polarity; female genitalia morphogenesis; G1 DNA damage checkpoint; gene expression; hair follicle morphogenesis; keratinocyte differentiation; keratinocyte proliferation; multicellular organismal aging; negative regulation of apoptosis; negative regulation of keratinocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuron apoptosis; Notch signaling pathway; odontogenesis of dentine-containing teeth; polarized epithelial cell differentiation; positive regulation of mesenchymal cell proliferation; positive regulation of Notch signaling pathway; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein homotetramerization; proximal/distal pattern formation; regulation of caspase activity; regulation of epidermal cell division; regulation of neuron apoptosis; replicative cell aging; response to DNA damage stimulus; response to gamma radiation; response to X-ray; skeletal development; skin morphogenesis; smooth muscle development; spermatogenesis; sympathetic nervous system development; transcription initiation from RNA polymerase II promoter; urinary bladder development
Disease: Adult Syndrome; Ankyloblepharon-ectodermal Defects-cleft Lip/palate; Ectrodactyly, Ectodermal Dysplasia, And Cleft Lip/palate Syndrome 3; Limb-mammary Syndrome; Rapp-hodgkin Syndrome; Split-hand/foot Malformation 4
Research Articles on p63
1. Delta Np63alpha-regulated microRNAs were found to regulate the levels of several metabolic factors
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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