AID: AID; ARP2; CDA2; HIGM2

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

1mg/ml (lot specific)

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-AICDA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]

Polyclonal

Western Blot (WB)

WB: 1:500 - 1:2000

Western blot analysis of extracts of Jurkat cells, using AICDA antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

198

activation-induced cytidine deaminase

single-stranded DNA cytosine deaminase; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos

Single-stranded DNA cytosine deaminase

AID??[Similar Products]

AICDA_HUMAN

This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]

AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family.

Protein type: EC 3.5.4.38; Hydrolase

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasm; exosome (RNase complex); nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; cytidine deaminase activity

Biological Process: somatic diversification of immunoglobulins; B cell differentiation; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing

Disease: Immunodeficiency With Hyper-igm, Type 2

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