For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Affinity Purified

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CYBA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This is a rabbit polyclonal antibody against CYBA. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated

Polyclonal; Growth Factors & Hormones; Membrane Protein; Drugs and Drug Metabolism; Disease Related;

Western Blot (WB), Immunohistochemistry (IHC)

Rabbit Anti-CYBA Antibody
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Plasma membrane in intercalated disk
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

WB Suggested Anti-CYBA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Small Intestine

21kDa

cytochrome b-245 alpha chain

cytochrome b-245 light chain

Cytochrome b-245 light chain

p22phox??[Similar Products]

CY24A_HUMAN

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]

CYBA: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. Defects in CYBA are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-negative (ARCGD). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Belongs to the p22phox family.

Protein type: Oxidoreductase; Membrane protein, integral; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; membrane; cell soma; mitochondrion; apical plasma membrane; dendrite; plasma membrane; endosome; secretory granule; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; SH3 domain binding

Biological Process: response to drug; respiratory burst; response to nutrient levels; interaction with host; angiotensin-mediated regulation of glomerular filtration; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of release of sequestered calcium ion into cytosol; antigen processing and presentation of peptide antigen via MHC class I; cytochrome complex assembly; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; smooth muscle hypertrophy; positive regulation of endothelial cell proliferation; inflammatory response; vascular endothelial growth factor receptor signaling pathway; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-negative

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