Full Product Name
CYBA siRNA (Human)
Product Synonym Names
Cytochrome b-245 light chain; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox
Product Gene Name
CYBA sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P13498
Specificity
CYBA siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CYBA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CYBA sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CYBA sirna
siRNA to inhibit CYBA expression using RNA interference
Applications Tested/Suitable for CYBA sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CYBA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000092.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000101.3
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UniProt Primary Accession #
P13498
[Other Products]
UniProt Secondary Accession #
Q14090; Q9BR72[Other Products]
UniProt Related Accession #
P13498[Other Products]
Molecular Weight
21,013 Da
NCBI Official Full Name
cytochrome b-245 light chain
NCBI Official Synonym Full Names
cytochrome b-245, alpha polypeptide
NCBI Official Symbol
CYBA??[Similar Products]
NCBI Official Synonym Symbols
p22-PHOX
??[Similar Products]
NCBI Protein Information
cytochrome b-245 light chain
UniProt Protein Name
Cytochrome b-245 light chain
UniProt Synonym Protein Names
Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox
Protein Family
Cytochrome
UniProt Gene Name
CYBA??[Similar Products]
UniProt Synonym Gene Names
p22phox??[Similar Products]
UniProt Entry Name
CY24A_HUMAN
NCBI Summary for CYBA
Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]
UniProt Comments for CYBA
CYBA: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. Defects in CYBA are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-negative (ARCGD). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Belongs to the p22phox family.
Protein type: Membrane protein, integral; Oxidoreductase; EC 1.-.-.-
Chromosomal Location of Human Ortholog: 16q24
Cellular Component: Golgi apparatus; phagocytic vesicle membrane; membrane; mitochondrion; cell soma; apical plasma membrane; dendrite; plasma membrane; endosome; secretory granule; NADPH oxidase complex
Molecular Function: protein binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; SH3 domain binding
Biological Process: respiratory burst; response to drug; response to nutrient levels; interaction with host; angiotensin-mediated regulation of glomerular filtration; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of release of sequestered calcium ion into cytosol; antigen processing and presentation of peptide antigen via MHC class I; cytochrome complex assembly; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; positive regulation of endothelial cell proliferation; smooth muscle hypertrophy; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process
Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-negative
Research Articles on CYBA
1. Data show that NADPH oxidase NOX4 and p22(phox) localize to the nuclear membrane in MV4-11 leukemia cells expressing internal tandem duplication of the FMS-like tyrosine kinase (FLT3-ITD) receptor.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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