Cytochrome b-245 light chain; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox; CYBA

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This CYBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the C-terminal region of human CYBA.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-CYBA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Cytochrome b is comprised of a light chain (alpha) and a
heavy chain (beta). This gene encodes the light, alpha subunit
which has been proposed as a primary component of the microbicidal
oxidase system of phagocytes. Mutations in this gene are associated
with autosomal recessive chronic granulomatous disease (CGD), that
is characterized by the failure of activated phagocytes to generate
superoxide, which is important for the microbicidal activity of
these cells.

Cancer; Cell Biology; Immunology; Metabolism; Neuroscience

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)

WB~~1:1000

CYBA Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CYBA antibody detected the CYBA protein (arrow).

Confocal immunofluorescent analysis of CYBA Antibody (C-term) with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

21013

cytochrome b-245, alpha polypeptide

cytochrome b-245 light chain

Cytochrome b-245 light chain

p22phox??[Similar Products]

CY24A_HUMAN

Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]

CYBA: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. Defects in CYBA are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-negative (ARCGD). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Belongs to the p22phox family.

Protein type: EC 1.-.-.-; Oxidoreductase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q24

Cellular Component: Golgi apparatus; phagocytic vesicle membrane; membrane; mitochondrion; cell soma; dendrite; apical plasma membrane; plasma membrane; endosome; secretory granule; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; SH3 domain binding

Biological Process: response to nutrient levels; response to drug; respiratory burst; interaction with host; angiotensin-mediated regulation of glomerular filtration; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of release of sequestered calcium ion into cytosol; antigen processing and presentation of peptide antigen via MHC class I; cytochrome complex assembly; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; smooth muscle hypertrophy; positive regulation of endothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-negative

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