Full Product Name
CYBB siRNA (Mouse)
Product Synonym Names
CGD; Cytochrome b-245 heavy chain; CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; Neutrophil cytochrome b 91 kDa polypeptide; gp91-1; gp91-phox; p22 phagocyte B-cytochrome
Product Gene Name
CYBB sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61093
Specificity
CYBB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CYBB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CYBB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CYBB sirna
siRNA to inhibit CYBB expression using RNA interference
Applications Tested/Suitable for CYBB sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CYBB. It may not necessarily be applicable to this product.
NCBI Accession #
NP_031833.3
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NCBI GenBank Nucleotide #
NM_007807.5
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UniProt Primary Accession #
Q61093
[Other Products]
UniProt Related Accession #
Q61093[Other Products]
Molecular Weight
65,305 Da
NCBI Official Full Name
cytochrome b-245 heavy chain
NCBI Official Synonym Full Names
cytochrome b-245, beta polypeptide
NCBI Official Symbol
Cybb??[Similar Products]
NCBI Official Synonym Symbols
Cgd; Cyd; Nox2; C88302; gp91-1; gp91phox; CGD91-phox
??[Similar Products]
NCBI Protein Information
cytochrome b-245 heavy chain
UniProt Protein Name
Cytochrome b-245 heavy chain
UniProt Synonym Protein Names
CGD91-phox; Cytochrome b(558) subunit beta; Cytochrome b558 subunit beta; Heme-binding membrane glycoprotein gp91phox; Neutrophil cytochrome b 91 kDa polypeptide; gp91-1; gp91-phox; p22 phagocyte B-cytochrome
Protein Family
Cytochrome
UniProt Gene Name
Cybb??[Similar Products]
UniProt Synonym Gene Names
Cgd; Cytochrome b558 subunit beta??[Similar Products]
UniProt Entry Name
CY24B_MOUSE
NCBI Summary for CYBB
This gene encodes the heavy chain component of a heterodimeric transmembrane ion transporter composed of both a heavy and a light chain. This transporter mediates the transfer of electrons from nicotinamide adenine dinucleotide phosphate (NADPH) to oxygen to generate superoxide. This reaction is important in the innate immune response to pathogens. However, increased activity of the encoded protein also leads to the generation of reactive oxygen species that result in oxidative stress and can cause tissue damage. Conversely, loss of function of the related gene in human causes chronic granulomatous disease. Alternative splicing results in multiple transcript variants, although the full-length nature of some of these variants has not been determined. [provided by RefSeq, May 2013]
UniProt Comments for CYBB
CYBB: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of granulomatous disease,chronic, X-linked (CGD). A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life- threatening bacterial/fungal infections. Defects in CYBB are a cause of mycobacteriosis atypical X-linked type 2 (AMCBX2). A rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections.
Protein type: Membrane protein, multi-pass; Oxidoreductase; Mitochondrial; Membrane protein, integral; EC 1.-.-.-
Cellular Component: Golgi apparatus; rough endoplasmic reticulum; mitochondrion; endoplasmic reticulum; integral to plasma membrane; dendrite; integral to membrane; membrane; cell soma; cytoplasm; plasma membrane; intracellular; NADPH oxidase complex
Molecular Function: protein binding; N-ethylmaleimide reductase activity; FAD binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; malolactic enzyme activity; voltage-gated ion channel activity; oxidoreductase activity; sulfur oxygenase reductase activity; F420H2 dehydrogenase activity
Biological Process: response to drug; respiratory burst; transport; superoxide metabolic process; innate immune response; ion transport; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process
Research Articles on CYBB
1. In chronic granulomatous disease, nox2-deficient B cells have a reduced capacity to kill bacteria.
Precautions
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Disclaimer
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