Product Name
AICDA, siRNA
Full Product Name
AICDA siRNA (Mouse)
Product Synonym Names
AID; Single-stranded DNA cytosine deaminase; Activation-induced cytidine deaminase; Cytidine aminohydrolase
Product Gene Name
AICDA sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9WVE0
Specificity
AICDA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AICDA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AICDA sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AICDA sirna
siRNA to inhibit AICDA expression using RNA interference
Applications Tested/Suitable for AICDA sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AICDA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_033775.1
[Other Products]
NCBI GenBank Nucleotide #
NM_009645.2
[Other Products]
UniProt Primary Accession #
Q9WVE0
[Other Products]
UniProt Related Accession #
Q9WVE0[Other Products]
Molecular Weight
24,031 Da
NCBI Official Full Name
single-stranded DNA cytosine deaminase
NCBI Official Synonym Full Names
activation-induced cytidine deaminase
NCBI Official Symbol
Aicda??[Similar Products]
NCBI Official Synonym Symbols
Aid; Arp2
??[Similar Products]
NCBI Protein Information
single-stranded DNA cytosine deaminase
UniProt Protein Name
Single-stranded DNA cytosine deaminase
UniProt Synonym Protein Names
Activation-induced cytidine deaminase; Cytidine aminohydrolase
Protein Family
Single-stranded DNA cytosine deaminase
UniProt Gene Name
Aicda??[Similar Products]
UniProt Synonym Gene Names
Aid??[Similar Products]
UniProt Entry Name
AICDA_MOUSE
UniProt Comments for AICDA
AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family.
Protein type: Hydrolase; EC 3.5.4.38
Cellular Component: cytoplasm; exosome (RNase complex); nucleus
Molecular Function: protein binding; zinc ion binding; hydrolase activity; ubiquitin protein ligase binding; metal ion binding; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines; catalytic activity; cytidine deaminase activity
Biological Process: somatic diversification of immunoglobulins; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing
Research Articles on AICDA
1. Observed an activity-dependent induction of BDNF exon IV mRNA expression, which correlated with reduced DNA methylation within the BDNF P4 promoter. Knockdown of AID disrupted these effects and inhibited BDNF exon IV mRNA expression.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
