Full Product Name
Anti-AICDA Antibody
Product Gene Name
anti-AICDA antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse. Not yet tested in other species.
Purity/Purification
Affinity purified
Concentration
100ug/100ul (lot specific)
Storage Buffer
PBS, pH 7.4 with 0.02% Sodium Azide
Immunogen
Rabbit polyclonal AICDA(1) antibody was raised against a 16 amino acid peptide corresponding to near the N terminal residues of Human AICDA.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Other Notes
Small volumes of anti-AICDA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AICDA antibody
The AICDA(Activation-induced cytidine deaminase)is a RNA-editing deaminase involved in somatic hypermutation,gene conversion, and class-switch recombination. It is required for several crucial steps of B-cell terminal differentiation necessaryfor efficient antibody responses. It belongs to the cytidine and deoxycytidylate deaminases family.
Applications Tested/Suitable for anti-AICDA antibody
ELISA (EIA), Western Blot (WB) Immunohistochemistry (IHC)
Application Notes for anti-AICDA antibody
Peptide ELISA titer: 1:1,000-1:10,000
Western blot analysis: 1:200-1000
Immunohistochemistry: 1:100-500
Optimal dilutions/concentrations should be determined by the end user
Western Blot (WB) of anti-AICDA antibody
Immunohistochemistry (IHC) #2 of anti-AICDA antibody
NCBI/Uniprot data below describe general gene information for AICDA. It may not necessarily be applicable to this product.
NCBI Accession #
AAU95780.1
[Other Products]
UniProt Secondary Accession #
Q6QJ81; Q8NFC1[Other Products]
UniProt Related Accession #
Q9GZX7[Other Products]
Molecular Weight
22,614 Da
NCBI Official Full Name
activation-induced deaminase, partial
NCBI Official Synonym Full Names
activation-induced cytidine deaminase
NCBI Official Symbol
AICDA??[Similar Products]
NCBI Official Synonym Symbols
AID; ARP2; CDA2; HIGM2; HEL-S-284
??[Similar Products]
NCBI Protein Information
single-stranded DNA cytosine deaminase; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos
UniProt Protein Name
Single-stranded DNA cytosine deaminase
UniProt Synonym Protein Names
Activation-induced cytidine deaminase; Cytidine aminohydrolase
Protein Family
Single-stranded DNA cytosine deaminase
UniProt Gene Name
AICDA??[Similar Products]
UniProt Entry Name
AICDA_HUMAN
NCBI Summary for AICDA
This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]
UniProt Comments for AICDA
AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family.
Protein type: EC 3.5.4.38; Hydrolase
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: cytoplasm; exosome (RNase complex); nucleus
Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; cytidine deaminase activity
Biological Process: somatic diversification of immunoglobulins; B cell differentiation; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing
Disease: Immunodeficiency With Hyper-igm, Type 2
Product References and Citations for anti-AICDA antibody
(1) Muto,T., Muramatsu,M., Taniwaki,M.et,al Isolation, tissue distribution, and chromosomal localization of the human activation-induced cytidine deaminase (AID) gene. Genomics.2000;68(1):85-88.
Research Articles on AICDA
1. AID activity and its relationship to chromosome folding and the B cell regulome was studied; find that AID-mediated lesions occur predominantly within B cell super-enhancers and regulatory clusters; further show that the structural and transcriptional features of these domains help explain AID tumorigenic activity in the B cell compartment of mice and humans.
Precautions
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Disclaimer
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