Full Product Name
EAAC1 Antibody, Clone S180-41: Dylight 633
Product Synonym Names
Mouse Anti-Human EAAC1 Monoclonal IgG1; EAAC1 Antibody: Dylight 633; Excitatory amino-acid carrier 1 Antibody; excitatory amino acid transporter 3 Antibody; EAAT3 Antibody; Neuronal and epithelial glutamate transporter Antibody; Sodium-dependent glutamate/aspartate transporter 3 Antibody; solute carrier family 1 member 1 and Slc1a1 Antibody
Product Gene Name
anti-EAAC1 antibody
[Similar Products]
Product Synonym Gene Name
EAAT3[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P43005
Species Reactivity
Human, Rat
Specificity
Detects ~60 kDa.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Fusion protein amino acids 1-524 (full length) of human EAAC1
Cellular Localization
Membrane
Certificate of Analysis
5 ug was sufficient for immunoprecipitation of EAAC1 from 50 ug of rat brain lysate.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, F?rster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-EAAC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-EAAC1 antibody
SLC1A1/EAAT3/EAAC1, a member of this transporter family, is expressed throughout the body, particularly in brain, intestine, liver, heart and kidney. Functional defects of the SLC1A1 gene are found in patients of dicarboxylicamino aciduria.
Product Categories/Family for anti-EAAC1 antibody
Neuroscience; Cell Markers; Neuron Markers; Membrane Markers; Pumps/Transporters; Neurotransmitter Receptors
Applications Tested/Suitable for anti-EAAC1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-EAAC1 antibody
WB: 1:1000
ICC/IF: 1:100
Immunocytochemistry (ICC) of anti-EAAC1 antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone S180-41 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) EAAC1 Antibody. (D) Composite.
Western Blot (WB) of anti-EAAC1 antibody
Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone S180-41 at 1:1000.
NCBI/Uniprot data below describe general gene information for EAAC1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_004161.4
[Other Products]
NCBI GenBank Nucleotide #
NP_004161.4
[Other Products]
UniProt Primary Accession #
P43005
[Other Products]
UniProt Related Accession #
P43005[Other Products]
NCBI Official Full Name
excitatory amino acid transporter 3
NCBI Official Synonym Full Names
solute carrier family 1 member 1
NCBI Official Symbol
SLC1A1??[Similar Products]
NCBI Official Synonym Symbols
DCBXA; EAAC1; EAAT3; SCZD18
??[Similar Products]
NCBI Protein Information
excitatory amino acid transporter 3
UniProt Protein Name
Excitatory amino acid transporter 3
UniProt Synonym Protein Names
Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1
UniProt Gene Name
SLC1A1??[Similar Products]
UniProt Synonym Gene Names
EAAC1; EAAT3??[Similar Products]
UniProt Entry Name
EAA3_HUMAN
NCBI Summary for EAAC1
This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain, these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate, and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate, and mutations in this gene are thought to cause dicarboxylicamino aciduria, also known as glutamate-aspartate transport defect. [provided by RefSeq, Mar 2010]
Product References and Citations for anti-EAAC1 antibody
1. Smith C. P., et al., (1994) Genomics 20:335-336. 2. Kanai Y., and Hediger M. A. (1992) Nature 360:467-471.
Research Articles on EAAC1
1. These preliminary results demonstrated that the SLC1A1 may contribute to some extent the susceptibility to obsessive-compulsive disorder and its symptoms
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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