Retinol-binding protein 3; Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein; RBP3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This RBP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 784-811 amino acids from the Central region of human RBP3.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-RBP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Interphotoreceptor retinol-binding protein is a large
glycoprotein known to bind retinoids and found primarily in the
interphotoreceptor matrix of the retina between the retinal pigment
epithelium and the photoreceptor cells. It is thought to transport
retinoids between the retinal pigment epithelium and the
photoreceptors, a critical role in the visual process.The human
IRBP gene is approximately 9.5 kbp in length and consists of four
exons separated by three introns. The introns are 1.6-1.9 kbp long.
The gene is transcribed by photoreceptor and retinoblastoma cells
into an approximately 4.3-kilobase mRNA that is translated and
processed into a glycosylated protein of 135,000 Da. The amino acid
sequence of human IRBP can be divided into four contiguous homology
domains with 33-38% identity, suggesting a series of gene
duplication events. In the gene, the boundaries of these domains
are not defined by exon-intron junctions, as might have been
expected. The first three homology domains and part of the fourth
are all encoded by the first large exon, which is 3,180 base pairs
long. The remainder of the fourth domain is encoded in the last
three exons, which are 191, 143, and approximately 740 base pairs
long, respectively.

Cardiovascular; Neuroscience; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

All lanes : Anti-RBP3 Antibody (Center) at 1:2000 dilution
Lane 1: mouse eyeball lysate Lane
2: mouse lung lysate Lysates/proteins at 20 ?g per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 135 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

135363

retinol binding protein 3, interstitial

retinol-binding protein 3

Retinol-binding protein 3

IRBP??[Similar Products]

RET3_HUMAN

Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively. [provided by RefSeq, Jul 2008]

RBP3: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. Belongs to the peptidase S41A family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; extracellular region; vesicle

Molecular Function: retinoid binding; serine-type peptidase activity; retinol binding; retinal binding

Biological Process: phototransduction, visible light; visual perception; transport; lipid metabolic process; retinoid metabolic process; proteolysis

Disease: Retinitis Pigmentosa 66; Retinitis Pigmentosa

Garcia-Ramirez, M., et al. Diabetologia 52(12):2633-2641(2009)
den Hollander, A.I., et al. Invest. Ophthalmol. Vis. Sci. 50(4):1864-1872(2009)
Jin, M., et al. J. Neurosci. 29(5):1486-1495(2009)
Descamps, F.J., et al. J. Cell. Mol. Med. 12 (6A), 2449-2456 (2008) :
Howard, O.M., et al. Blood 105(11):4207-4214(2005)

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