Full Product Name
BSND siRNA (Mouse)
Product Synonym Names
Barttin
Product Gene Name
BSND sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8VIM4
Specificity
BSND siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BSND gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of BSND sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BSND sirna
siRNA to inhibit BSND expression using RNA interference
Applications Tested/Suitable for BSND sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for BSND. It may not necessarily be applicable to this product.
NCBI Accession #
NP_536706.2
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NCBI GenBank Nucleotide #
NM_080458.2
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UniProt Primary Accession #
Q8VIM4
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UniProt Secondary Accession #
Q8C740; Q8CHY0; B1AZI5[Other Products]
UniProt Related Accession #
Q8VIM4[Other Products]
Molecular Weight
33,813 Da
NCBI Official Full Name
barttin
NCBI Official Synonym Full Names
Bartter syndrome, infantile, with sensorineural deafness (Barttin)
NCBI Official Symbol
Bsnd??[Similar Products]
NCBI Protein Information
barttin
UniProt Protein Name
Barttin
UniProt Gene Name
Bsnd??[Similar Products]
UniProt Entry Name
BSND_MOUSE
UniProt Comments for BSND
BSND: Functions as a beta-subunit for CLCNKA and CLCNKB chloride channels. In the kidney CLCNK/BSND heteromers mediate chloride reabsorption by facilitating its basolateral efflux. In the stria, CLCNK/BSND channels drive potassium secretion by recycling chloride for the basolateral SLC12A2 cotransporter. Defects in BSND are the cause of Bartter syndrome type 4A (BS4A); also known as infantile Bartter syndrome with sensorineural deafness. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS4A is associated with sensorineural deafness.
Protein type: Transporter; Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel
Cellular Component: protein complex; membrane; cell; basolateral plasma membrane; integral to plasma membrane; integral to membrane; plasma membrane
Molecular Function: chloride channel activity; protein binding; chloride channel regulator activity
Biological Process: sensory perception of sound; cellular ion homeostasis; chloride transport; cellular chloride ion homeostasis; cellular potassium ion homeostasis
Research Articles on BSND
1. induction of SGK1, CLC-K1 and barttin by high osmolarity and change in intracellular volume in distal renal tubular cells in vivo and in vitro
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
