Full Product Name
RBP3 siRNA (Human)
Product Synonym Names
Retinol-binding protein 3; Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein
Product Gene Name
RBP3 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P10745
Specificity
RBP3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RBP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RBP3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RBP3 sirna
siRNA to inhibit RBP3 expression using RNA interference
Applications Tested/Suitable for RBP3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RBP3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002891.1
[Other Products]
NCBI GenBank Nucleotide #
NM_002900.2
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UniProt Primary Accession #
P10745
[Other Products]
UniProt Secondary Accession #
Q0QD34; Q5VSR0; Q8IXN0[Other Products]
UniProt Related Accession #
P10745[Other Products]
Molecular Weight
135,363 Da
NCBI Official Full Name
retinol-binding protein 3
NCBI Official Synonym Full Names
retinol binding protein 3, interstitial
NCBI Official Symbol
RBP3??[Similar Products]
NCBI Official Synonym Symbols
IRBP; RBPI; RP66; D10S64; D10S65; D10S66
??[Similar Products]
NCBI Protein Information
retinol-binding protein 3
UniProt Protein Name
Retinol-binding protein 3
UniProt Synonym Protein Names
Interphotoreceptor retinoid-binding protein; IRBP; Interstitial retinol-binding protein
Protein Family
Retinol-binding protein
UniProt Gene Name
RBP3??[Similar Products]
UniProt Synonym Gene Names
IRBP??[Similar Products]
UniProt Entry Name
RET3_HUMAN
NCBI Summary for RBP3
Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively. [provided by RefSeq, Jul 2008]
UniProt Comments for RBP3
RBP3: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. Belongs to the peptidase S41A family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q11.2
Cellular Component: extracellular space; extracellular region; vesicle
Molecular Function: retinoid binding; serine-type peptidase activity; retinol binding; retinal binding
Biological Process: phototransduction, visible light; visual perception; transport; lipid metabolic process; proteolysis; retinoid metabolic process
Disease: Retinitis Pigmentosa 66; Retinitis Pigmentosa
Research Articles on RBP3
1. CMPK1 and RBP3 are associated with corneal curvature in Asian populations.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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