Full Product Name
DYM siRNA (Human)
Product Synonym Names
Dymeclin; Dyggve-Melchior-Clausen syndrome protein
Product Gene Name
DYM sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7RTS9
Specificity
DYM siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DYM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DYM sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DYM sirna
siRNA to inhibit DYM expression using RNA interference
Applications Tested/Suitable for DYM sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DYM. It may not necessarily be applicable to this product.
NCBI Accession #
NP_060123.3
[Other Products]
NCBI GenBank Nucleotide #
NM_017653.3
[Other Products]
UniProt Primary Accession #
Q7RTS9
[Other Products]
UniProt Secondary Accession #
Q3ZTS8; Q6P2P5; Q8N2M0; Q9BVE9; Q9NPU7; A8K5I8; B2RCF9; B4DKI7[Other Products]
UniProt Related Accession #
Q7RTS9[Other Products]
Molecular Weight
54,425 Da
NCBI Official Full Name
dymeclin
NCBI Official Synonym Full Names
dymeclin
NCBI Official Symbol
DYM??[Similar Products]
NCBI Official Synonym Symbols
DMC; SMC
??[Similar Products]
NCBI Protein Information
dymeclin
UniProt Protein Name
Dymeclin
UniProt Synonym Protein Names
Dyggve-Melchior-Clausen syndrome protein
UniProt Gene Name
DYM??[Similar Products]
UniProt Entry Name
DYM_HUMAN
NCBI Summary for DYM
This gene encodes a protein which is necessary for normal skeletal development and brain function. Mutations in this gene are associated with two types of recessive osteochondrodysplasia, Dyggve-Melchior-Clausen (DMC) dysplasia and Smith-McCort (SMC) dysplasia, which involve both skeletal defects and mental retardation. [provided by RefSeq, Jul 2008]
UniProt Comments for DYM
DYM: Necessary for correct organization of Golgi apparatus. Involved in bone development. Defects in DYM are the cause of Dyggve-Melchior-Clausen syndrome (DMC). DMC is a rare autosomal recessive disorder characterized by short trunk dwarfism, microcephaly and psychomotor retardation. Electron microscopic study of cutaneous cells of affected patients shows dilated rough endoplasmic reticulum, enlarged and aberrant vacuoles and numerous vesicles. DMC is progressive. Defects in DYM are the cause of Smith-McCort dysplasia (SMC). SMC is a rare autosomal recessive osteochondrodysplasia characterized by short limbs and trunk with barrel-shaped chest. The radiographic phenotype includes platyspondyly, generalized abnormalities of the epiphyses and metaphyses, and a distinctive lacy appearance of the iliac crest, features identical to those of Dyggve-Melchior-Clausen syndrome. Belongs to the dymeclin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 18q21.1
Cellular Component: Golgi apparatus; cytoplasm
Molecular Function: protein binding; enzyme binding
Biological Process: Golgi organization and biogenesis
Disease: Dyggve-melchior-clausen Disease; Smith-mccort Dysplasia 1
Research Articles on DYM
1. T (p.R194X).">The clinical diagnosis was confirmed with molecular analysis of DYM with a known mutation at c.580C>T (p.R194X).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
