CYBB; cytochrome b-245, beta polypeptide (chronic granulomatous disease); CGD; GP91-1; GP91-PHOX; GP91PHOX; NOX2; cytochrome b-245, beta polypeptide; CYBB antibody; cytochrome b-245; beta polypeptide (chronic granulomatous disease) antibody; CGD antibody; GP91-1 antibody; GP91-PHOX antibody; GP91PHOX antibody; NOX2 antibody; cytochrome b-245; beta polypeptide antibody; CYBB / GP91-PHOX

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-CYBB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 2000.
Immunohistochemistry: In paraffin embedded Human Spleen shows staining of select cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 85Da band observed in Human Peripheral Blood Mononucleocytes ysates (calculated MW of 65.3kDa according to NP_000388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Teahan et al, Nature.1987 Jun 25-Jul 1;327(6124): 720-1. PMID: 3600769). Recommended concentration: 0. 03-0.1ug/ml.

(0. 03ug/ml) staining of Human Peripheral Blood Mononucleocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

(2ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

65,336 Da

cytochrome b-245 beta chain

cytochrome b-245 heavy chain

Cytochrome b-245 heavy chain

NOX2; Cytochrome b558 subunit beta??[Similar Products]

CY24B_HUMAN

Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]

CYBB: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of granulomatous disease,chronic, X-linked (CGD). A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life- threatening bacterial/fungal infections. Defects in CYBB are a cause of mycobacteriosis atypical X-linked type 2 (AMCBX2). A rare condition characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections.

Protein type: EC 1.-.-.-; Mitochondrial; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase

Chromosomal Location of Human Ortholog: Xp21.1

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; mitochondrion; NADPH oxidase complex; phagocytic vesicle membrane; plasma membrane

Molecular Function: electron carrier activity; FAD binding; heme binding; metal ion binding; protein binding; protein heterodimerization activity; superoxide-generating NADPH oxidase activity; voltage-gated ion channel activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; hydrogen peroxide biosynthetic process; inflammatory response; innate immune response; ion transport; respiratory burst; response to reactive oxygen species; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, X-linked; Immunodeficiency 34

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