Anti -AP-2 beta

For Research Use Only. Not for use in diagnostic procedures.

chromosome: 6; Location: 6p12

Polyclonal

IgG

Rabbit

Detects endogenous levels of total human AP-2 beta protein at 49kD. Species Crossreactivity: mouse and rat.

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-TFAP2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The sequence-specific transcription factor activator protein 2 beta (AP-2 beta) is required for normal kidney development (1). AP-2 beta knockout mice die 1-2 days after birth due to polycystic kidney disease (1). Massive apoptosis occured during kidney deveopment at the end of embryogenesis in these mice (1). Overexpressed AP-2 beta has been to found to suppress c-myc-induced apoptosis, indicating a role of this transcription factor in cell survival (1). In addition, overexpression of AP-2 beta is shown to be related to impaired insulin signaling in adipocytes, and is therefore proposed to be a candidate gene that may relate to obesity (2).

Antibodies; Abs to Transcription Factors

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation.
Dilution: Western Blot 1:1000
Immunoprecipitation 1:100
Immunofluorescence (IF-IC) 1:100

50,474 Da[Similar Products]

transcription factor AP-2 beta (activating enhancer binding protein 2 beta)

transcription factor AP-2-beta; AP2-beta; activating enhancer binding protein 2 beta; activating enhancer-binding protein 2-beta

Transcription factor AP-2-beta

AP2B_HUMAN

This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives. [provided by RefSeq]

AP-2 beta: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia. Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with CITED4. Interacts with UBE2I. Interacts with KCTD1; this interaction represses transcription activation. Interacts with CITED2 (via C-terminus); the interaction stimulates TFAP2B-transcriptional activity. Belongs to the AP-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; caspase inhibitor activity; chromatin binding; transcription corepressor activity; transcription factor activity

Biological Process: skin development; fat cell differentiation; transcription from RNA polymerase II promoter; hindlimb morphogenesis; response to lithium ion; positive regulation of transcription, DNA-dependent; regulation of BMP signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; glucose homeostasis; calcium ion homeostasis; forelimb morphogenesis; negative regulation of cell proliferation; sympathetic nervous system development; regulation of transcription, DNA-dependent; regulation of cell differentiation; positive regulation of neuron apoptosis; positive regulation of cell proliferation; sodium ion homeostasis; phosphate ion homeostasis; negative regulation of neuron apoptosis; kidney development; potassium ion homeostasis; response to drug; glucose metabolic process; renal water homeostasis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of insulin secretion; negative regulation of apoptosis

Disease: Char Syndrome

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