Full Product Name
Goat anti-NEFL (aa330-343) Antibody
Product Synonym Names
NEFL; neurofilament, light polypeptide; CMT1F; CMT2E; NF-L; NF68; NFL; PPP1R110; light molecular weight neurofilament protein; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; protein phosphatase 1, regulatory subunit 110
Product Gene Name
anti-NEFL antibody
[Similar Products]
Antibody/Peptide Pairs
NEFL peptide (MBS428859) is used for blocking the activity of NEFL antibody (MBS423821)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P07196
Species Reactivity
Human
Expected from sequence similarity: Mouse, Rat, Dog, Pig, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen Sequence
Peptide with sequence C-EKQLQELEDKQNAD, from the internal region of the protein sequence according to NP_006149.2.
Peptide Sequence + C
C-EKQLQELEDKQNAD
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-NEFL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-NEFL antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-NEFL antibody
Pep-ELISA: antibody detection limit dilution 1:4000.
WB: Approx 68-70kDa band observed in lysates of Neuroblastoma cell line KELLY. Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry (IHC) of anti-NEFL antibody
(1ug/ml) staining of Neuroblastoma KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for NEFL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006149.2
[Other Products]
NCBI GenBank Nucleotide #
NM_006158.4
[Other Products]
UniProt Primary Accession #
P07196
[Other Products]
UniProt Secondary Accession #
Q16154; Q8IU72; B9ZVN2[Other Products]
UniProt Related Accession #
P07196[Other Products]
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament light
NCBI Official Symbol
NEFL??[Similar Products]
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110
??[Similar Products]
NCBI Protein Information
neurofilament light polypeptide
UniProt Protein Name
Neurofilament light polypeptide
UniProt Synonym Protein Names
68 kDa neurofilament protein; Neurofilament triplet L protein
Protein Family
Neurofilament light polypeptide
UniProt Gene Name
NEFL??[Similar Products]
UniProt Synonym Gene Names
NF68; NFL; NF-L??[Similar Products]
NCBI Summary for NEFL
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
UniProt Comments for NEFL
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Research Articles on NEFL
1. CSF neurofilament light chain protein is an accurate marker for distinguishing Alzheimer's disease patients from healthy controls.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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