Product Name
AVPR2, siRNA
Full Product Name
AVPR2 siRNA (Mouse)
Product Synonym Names
V2R; Vasopressin V2 receptor; V2R; AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor
Product Gene Name
AVPR2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O88721
Specificity
AVPR2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AVPR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of AVPR2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AVPR2 sirna
siRNA to inhibit AVPR2 expression using RNA interference
Applications Tested/Suitable for AVPR2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for AVPR2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_062277.1
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NCBI GenBank Nucleotide #
NM_019404.2
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UniProt Primary Accession #
O88721
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UniProt Related Accession #
O88721[Other Products]
Molecular Weight
40,645 Da
NCBI Official Full Name
vasopressin V2 receptor isoform a
NCBI Official Synonym Full Names
arginine vasopressin receptor 2
NCBI Official Symbol
Avpr2??[Similar Products]
NCBI Official Synonym Symbols
DI1; DIR; ND1; V2R; ADHR; VPV2R
??[Similar Products]
NCBI Protein Information
vasopressin V2 receptor
UniProt Protein Name
Vasopressin V2 receptor
UniProt Synonym Protein Names
AVPR V2; Antidiuretic hormone receptor; Renal-type arginine vasopressin receptor
Protein Family
Vasopressin V2 receptor
UniProt Gene Name
Avpr2??[Similar Products]
UniProt Synonym Gene Names
V2r; V2R??[Similar Products]
UniProt Entry Name
V2R_MOUSE
NCBI Summary for AVPR2
This gene encodes a member of the G-protein coupled receptor 1 family and the vasopressin/oxytocin receptor subfamily. The encoded protein is an arginine vasopressin receptor which, when stimulated, activates the Gs protein/adenylyl cyclase signaling cascade and is involved in water and electrolyte homeostasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
UniProt Comments for AVPR2
AVPR2: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Involved in renal water reabsorption. Defects in AVPR2 are the cause of nephrogenic syndrome of inappropriate antidiuresis (NSIAD). This disorder is characterized by an inability to excrete a free water load, with inappropriately concentrated urine and resultant hyponatremia, hypoosmolarity, and natriuresis. Defects in AVPR2 are the cause of diabetes insipidus nephrogenic X-linked (XNDI); also known as diabetes insipidus nephrogenic type 1. XNDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; Transporter; Transporter, aquaporin family
Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane; intracellular
Molecular Function: G-protein coupled receptor activity; signal transducer activity; peptide binding; vasopressin receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; I-kappaB kinase/NF-kappaB cascade; regulation of systemic arterial blood pressure by vasopressin; response to cytokine stimulus; positive regulation of cell proliferation; interferon-gamma production; positive regulation of vasoconstriction; positive regulation of systemic arterial blood pressure; signal transduction; positive regulation of blood pressure; cellular response to hormone stimulus
Research Articles on AVPR2
1. In polycystic kidney disease, structural or functional loss of cilia leads to abnormal trafficking of AQP2/V2R leading to enhanced salt and water absorption.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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