Product Name
SCN5A, siRNA
Full Product Name
SCN5A siRNA (Rat)
Product Synonym Names
Sodium channel protein type 5 subunit alpha; Sodium channel protein cardiac muscle subunit alpha; Sodium channel protein type V subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.5
Product Gene Name
SCN5A sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15389
Specificity
SCN5A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SCN5A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SCN5A sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SCN5A sirna
siRNA to inhibit SCN5A expression using RNA interference
Applications Tested/Suitable for SCN5A sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SCN5A. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001153634.1
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NCBI GenBank Nucleotide #
NM_001160162.1
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UniProt Primary Accession #
P15389
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UniProt Secondary Accession #
Q925G6[Other Products]
UniProt Related Accession #
P15389[Other Products]
Molecular Weight
221,706 Da
NCBI Official Full Name
sodium channel protein type 5 subunit alpha isoform 2
NCBI Official Synonym Full Names
sodium channel, voltage-gated, type V, alpha subunit
NCBI Official Symbol
Scn5a??[Similar Products]
NCBI Official Synonym Symbols
SCAL; Nav1.5
??[Similar Products]
NCBI Protein Information
sodium channel protein type 5 subunit alpha
UniProt Protein Name
Sodium channel protein type 5 subunit alpha
UniProt Synonym Protein Names
Sodium channel protein cardiac muscle subunit alpha; Sodium channel protein type V subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.5
Protein Family
Sodium channel protein
UniProt Gene Name
Scn5a??[Similar Products]
UniProt Entry Name
SCN5A_RAT
NCBI Summary for SCN5A
acts as a tetrodotoxin-resistant voltage gated sodium channel; may play a role in cardiac function [RGD, Feb 2006]
UniProt Comments for SCN5A
SCN5A: an integral membrane protein and a voltage-gated sodium channel subunit. Mediates the voltage-dependent sodium ion permeability of excitable membranes. This protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant Na(+) channel isoform. Intracellular calcium levels regulate channel inactivation. Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2. Interacts with NEDD4, NEDD4L, WWP2 and GPD1L. Defects in this protein are a cause of long QT syndrome type 3 (LQT3). Belongs to the sodium channel (TC 1.A.1.10) family, Nav1.5/SCN5A subfamily. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Channel, sodium; Membrane protein, multi-pass; Membrane protein, integral
Cellular Component: voltage-gated sodium channel complex; cell surface; endoplasmic reticulum; T-tubule; plasma membrane; integral to membrane; caveola; sarcolemma
Molecular Function: calmodulin binding; protein binding; fibroblast growth factor binding; enzyme binding; ubiquitin protein ligase binding; voltage-gated sodium channel activity; ankyrin binding; nitric-oxide synthase binding; protein kinase binding
Biological Process: regulation of heart rate; sodium ion transport; generation of action potential; positive regulation of heart rate; response to denervation involved in regulation of muscle adaptation; response to organic cyclic substance; odontogenesis of dentine-containing teeth; membrane depolarization; telencephalon development; cerebellum development; positive regulation of action potential; positive regulation of epithelial cell proliferation; cardiac muscle contraction
Research Articles on SCN5A
1. Provocation of an autoimmune response against NaV1.5 induces cardiac conduction defects.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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