Full Product Name
SCO2 siRNA (Mouse)
Product Synonym Names
Protein SCO2 homolog mitochondrial
Product Gene Name
SCO2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8VCL2
Specificity
SCO2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SCO2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SCO2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SCO2 sirna
siRNA to inhibit SCO2 expression using RNA interference
Applications Tested/Suitable for SCO2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SCO2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001104758.1
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NCBI GenBank Nucleotide #
NM_001111288.1
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UniProt Primary Accession #
Q8VCL2
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UniProt Related Accession #
Q8VCL2[Other Products]
Molecular Weight
28,944 Da
NCBI Official Full Name
protein SCO2 homolog, mitochondrial
NCBI Official Synonym Full Names
SCO cytochrome oxidase deficient homolog 2 (yeast)
NCBI Official Symbol
Sco2??[Similar Products]
NCBI Protein Information
protein SCO2 homolog, mitochondrial
UniProt Protein Name
Protein SCO2 homolog, mitochondrial
Protein Family
SCO2-like protein
UniProt Gene Name
Sco2??[Similar Products]
UniProt Entry Name
SCO2_MOUSE
UniProt Comments for SCO2
SCO2: Acts as a copper chaperone, transporting copper to the Cu(A) site on the cytochrome c oxidase subunit II (COX2)
Protein type: Mitochondrial
Cellular Component: nucleoplasm; myofibril; mitochondrion; mitochondrial inner membrane
Molecular Function: copper ion binding; metal ion binding
Biological Process: cellular copper ion homeostasis; eye development; in utero embryonic development; respiratory chain complex IV assembly; response to activity; copper ion transport; muscle system process; copper ion homeostasis
Research Articles on SCO2
1. Data suggest that physical exercise attenuates age-related changes in mitochondrial COX biogenesis and p53 activity targeting SCO2 and mitochondria, and thereby induces antisenescent and protective effects in cardiac muscle.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
