For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C

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Small volumes of SCN5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9904174 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sodium Channel Protein Type 5 Subunit Alpha (SCN5A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SCN5A. The ELISA analytical biochemical technique of the MBS9904174 kit is based on SCN5A antibody-SCN5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SCN5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SCN5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

224,917 Da

sodium voltage-gated channel alpha subunit 5

sodium channel protein type 5 subunit alpha

Sodium channel protein type 5 subunit alpha

The protein encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. This protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene are a cause of long QT syndrome type 3 (LQT3), an autosomal dominant cardiac disease. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

SCN5A: an integral membrane protein and a voltage-gated sodium channel subunit. Mediates the voltage-dependent sodium ion permeability of excitable membranes. This protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant Na(+) channel isoform. Intracellular calcium levels regulate channel inactivation. Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2. Interacts with NEDD4, NEDD4L, WWP2 and GPD1L. Defects in this protein are a cause of long QT syndrome type 3 (LQT3). Belongs to the sodium channel (TC 1.A.1.10) family, Nav1.5/SCN5A subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, sodium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p22.2

Cellular Component: caveola; cell surface; endoplasmic reticulum; integral component of membrane; intercalated disc; intracellular; lateral plasma membrane; plasma membrane; sarcolemma; T-tubule; voltage-gated sodium channel complex; Z disc

Molecular Function: ankyrin binding; calmodulin binding; enzyme binding; fibroblast growth factor binding; ion channel binding; nitric-oxide synthase binding; protein binding; protein domain specific binding; protein kinase binding; ubiquitin protein ligase binding; voltage-gated ion channel activity; voltage-gated sodium channel activity; voltage-gated sodium channel activity involved in AV node cell action potential; voltage-gated sodium channel activity involved in bundle of His cell action potential; voltage-gated sodium channel activity involved in cardiac muscle cell action potential; voltage-gated sodium channel activity involved in Purkinje myocyte action potential; voltage-gated sodium channel activity involved in SA node cell action potential

Biological Process: atrial cardiac muscle cell action potential; AV node cell action potential; AV node cell to bundle of His cell communication; brainstem development; bundle of His cell action potential; cardiac muscle cell action potential involved in contraction; cardiac muscle contraction; cardiac ventricle development; cellular response to calcium ion; cerebellum development; generation of action potential; membrane depolarization; membrane depolarization during action potential; membrane depolarization during atrial cardiac muscle cell action potential; membrane depolarization during AV node cell action potential; membrane depolarization during bundle of His cell action potential; membrane depolarization during cardiac muscle cell action potential; membrane depolarization during Purkinje myocyte cell action potential; membrane depolarization during SA node cell action potential; odontogenesis of dentine-containing teeth; positive regulation of action potential; positive regulation of epithelial cell proliferation; positive regulation of sodium ion transport; regulation of cardiac muscle cell contraction; regulation of heart rate; regulation of heart rate by cardiac conduction; regulation of sodium ion transmembrane transport; response to denervation involved in regulation of muscle adaptation; SA node cell action potential; sodium ion transport; telencephalon development; ventricular cardiac muscle cell action potential

Disease: Atrial Fibrillation, Familial, 10; Brugada Syndrome 1; Cardiomyopathy, Dilated, 1e; Long Qt Syndrome 3; Progressive Familial Heart Block, Type Ia; Sick Sinus Syndrome 1, Autosomal Recessive; Sudden Infant Death Syndrome; Ventricular Fibrillation During Myocardial Infarction, Susceptibility To

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