Full Product Name
Goat anti-Alk (mouse) Antibody
Product Synonym Names
Goat Anti-ALK Antibody; Alk; anaplastic lymphoma receptor tyrosine kinase; CD246; NBLST3; ALK tyrosine kinase receptor; CD246 antigen; Tcrz; ALK tyrosine kinase receptor antibody; anaplastic lymphoma receptor tyrosine kinase antibody; CD246 antibody; CD246 antigen antibody; NBLST3 antibody; Tcrz antibody; Alk antibody; Alk (mouse)
Product Gene Name
anti-Alk antibody
[Similar Products]
Antibody/Peptide Pairs
Alk peptide (MBS427620) is used for blocking the activity of Alk antibody (MBS422683)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
TETFHPERLE SNS
Species Reactivity
Tested: Rat; Expected from sequence similarity: Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-TETFHPERLESNS, from the internal region of the protein sequence according to NP_031465.2.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-Alk antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Alk antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-Alk antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 150kDa band observed in Rat Brain lysates (calculated MW of 175kDa according to Rat NP_001162572.1). Recommended concentration: 0. 03-0.1ug/ml.
Western Blot (WB) of anti-Alk antibody
(0. 03ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for Alk. It may not necessarily be applicable to this product.
NCBI Accession #
NP_031465.2
[Other Products]
NCBI GenBank Nucleotide #
NM_007439.2
[Other Products]
UniProt Secondary Accession #
E9QKV3[Other Products]
UniProt Related Accession #
P97793[Other Products]
Molecular Weight
174,948 Da
NCBI Official Full Name
ALK tyrosine kinase receptor
NCBI Official Synonym Full Names
anaplastic lymphoma kinase
NCBI Official Symbol
Alk??[Similar Products]
NCBI Official Synonym Symbols
Tcrz; CD246
??[Similar Products]
NCBI Protein Information
ALK tyrosine kinase receptor
UniProt Protein Name
ALK tyrosine kinase receptor
UniProt Synonym Protein Names
Anaplastic lymphoma kinase; CD_antigen: CD246
Protein Family
Alkylsulfatase
UniProt Gene Name
Alk??[Similar Products]
UniProt Entry Name
ALK_MOUSE
UniProt Comments for Alk
ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors
Protein type: Oncoprotein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Kinase, protein; TK group; Alk family
Cellular Component: axon; integral to membrane; integral to plasma membrane; membrane; plasma membrane; protein complex
Molecular Function: ATP binding; identical protein binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity; transmembrane receptor protein tyrosine kinase activity
Biological Process: activation of MAPK activity; nervous system development; neuron development; peptidyl-tyrosine phosphorylation; phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on Alk
1. ALK knock out male mice exhibit hypogonadotropic hypogonadism.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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