Product Name
KCNE3, siRNA
Full Product Name
KCNE3 siRNA (Rat)
Product Synonym Names
Potassium voltage-gated channel subfamily E member 3; MinK-related peptide 2; Minimum potassium ion channel-related peptide 2; Potassium channel subunit beta MiRP2
Product Gene Name
KCNE3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JJV7
Specificity
KCNE3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat KCNE3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KCNE3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KCNE3 sirna
siRNA to inhibit KCNE3 expression using RNA interference
Applications Tested/Suitable for KCNE3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KCNE3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_071571.1
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NCBI GenBank Nucleotide #
NM_022235.2
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UniProt Primary Accession #
Q9JJV7
[Other Products]
UniProt Related Accession #
Q9JJV7[Other Products]
Molecular Weight
12,001 Da
NCBI Official Full Name
potassium voltage-gated channel subfamily E member 3
NCBI Official Synonym Full Names
potassium channel, voltage-gated Isk-related subfamily E regulatory beta subunit 3
NCBI Official Symbol
Kcne3??[Similar Products]
NCBI Protein Information
potassium voltage-gated channel subfamily E member 3
UniProt Protein Name
Potassium voltage-gated channel subfamily E member 3
UniProt Synonym Protein Names
MinK-related peptide 2; Minimum potassium ion channel-related peptide 2; Potassium channel subunit beta MiRP2
Protein Family
Potassium voltage-gated channel subfamily
UniProt Gene Name
Kcne3??[Similar Products]
UniProt Entry Name
KCNE3_RAT
NCBI Summary for KCNE3
a regulator that is coexpressed with K+ channel KCNQ1 [RGD, Feb 2006]
UniProt Comments for KCNE3
Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 (PubMed:12954870). Associated with KCNC4/Kv3.4 is proposed to form the subthreshold voltage-gated potassium channel in skeletal muscle and to establish the resting membrane potential (RMP) in muscle cells (). Associated with KCNQ1/KCLQT1 may form the intestinal cAMP-stimulated potassium channel involved in chloride secretion that produces a current with nearly instantaneous activation with a linear current-voltage relationship (PubMed:21911611).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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