Anti -Acetyl CoA Carboxylase 1 (ACAC, ACC, ACC1, ACCA, ACC-alpha, Acetyl-CoA carboxylase alpha)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 17; NC_000017.10 (35441927..35766902, complement). Location: 17q21

Monoclonal

IgG

7i5

Rabbit

Recognizes human Acetyl CoA Carboxylase 1 at ~265kD. Species Crossreactivity: mouse and rat.

Purified
Purified

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, before the addition of glycerol to 40%.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-ACACA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Abs to Enzymes

Western Blot (WB), Immunohistochemistry (IHC)

Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:500-1:1000 detected endogenous Acetyl CoA Carboxylase in lysates from A431 cells. Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.
Immunohistochemistry: 1:50 dilution detected Acetyl CoA Carboxylase in paraffin-embedded human liver.

265,554 Da[Similar Products]

acetyl-CoA carboxylase alpha

acetyl-CoA carboxylase 1; ACC-alpha; OTTHUMP00000164069; OTTHUMP00000164070; OTTHUMP00000164076; acetyl-Coenzyme A carboxylase alpha

Acetyl-CoA carboxylase 1

ACAC; ACC1; ACCA??[Similar Products]

ACACA_HUMAN

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq]

ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity.

Protein type: Ligase; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; EC 6.4.1.2; EC 6.3.4.14; Lipid Metabolism - fatty acid biosynthesis

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: mitochondrion; cytoplasm; nucleolus; cytosol; actin cytoskeleton

Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding

Biological Process: vitamin metabolic process; acetyl-CoA metabolic process; positive regulation of cellular metabolic process; lipid homeostasis; triacylglycerol biosynthetic process; carnitine shuttle; cellular lipid metabolic process; biotin metabolic process; protein homotetramerization; multicellular organismal protein metabolic process; energy reserve metabolic process; tissue homeostasis; water-soluble vitamin metabolic process; fatty acid biosynthetic process

Disease: Acetyl-coa Carboxylase Deficiency

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