CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of High Sensitive Neurofilament, Light Polypeptide (NEFL).
No significant cross-reactivity or interference between High Sensitive Neurofilament, Light Polypeptide (NEFL) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NEFL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2087077 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurofilament, Light Polypeptide (NEFL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NEFL. The ELISA analytical biochemical technique of the MBS2087077 kit is based on NEFL antibody-NEFL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NEFL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NEFL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Neurofilament, Light Polypeptide (NEFL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Neurofilament, Light Polypeptide (NEFL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Neurofilament, Light Polypeptide (NEFL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Neuroscience

61,508 Da

neurofilament, light polypeptide

neurofilament light polypeptide

Neurofilament light polypeptide

Nf68; Nfl; NF-L??[Similar Products]

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 14|14 D1

Cellular Component: axon; cytoplasm; growth cone; intermediate filament; myelin sheath; neurofilament; neuron projection; TSC1-TSC2 complex

Molecular Function: identical protein binding; phospholipase binding; protein binding; protein binding, bridging; protein C-terminus binding; protein domain specific binding; protein heterodimerization activity; structural constituent of cytoskeleton

Biological Process: anterograde axon cargo transport; axon regeneration in the peripheral nervous system; axon transport of mitochondrion; intermediate filament bundle assembly; intermediate filament organization; intermediate filament polymerization and/or depolymerization; locomotion; microtubule cytoskeleton organization and biogenesis; negative regulation of neuron apoptosis; neurite morphogenesis; neurofilament bundle assembly; neurofilament cytoskeleton organization and biogenesis; neuromuscular process controlling balance; positive regulation of axonogenesis; protein polymerization; regulation of axon diameter; retrograde axon cargo transport

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