Full Product Name
NEK1 siRNA (Mouse)
Product Synonym Names
Serine/threonine-protein kinase Nek1; Never in mitosis A-related kinase 1; NimA-related protein kinase 1
Product Gene Name
NEK1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P51954
Specificity
NEK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NEK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NEK1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NEK1 sirna
siRNA to inhibit NEK1 expression using RNA interference
Applications Tested/Suitable for NEK1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NEK1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001280566.1
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NCBI GenBank Nucleotide #
NM_001293637.1
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UniProt Primary Accession #
P51954
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UniProt Related Accession #
P51954[Other Products]
Molecular Weight
136,690 Da
NCBI Official Full Name
serine/threonine-protein kinase Nek1 isoform 1
NCBI Official Synonym Full Names
NIMA (never in mitosis gene a)-related expressed kinase 1
NCBI Official Symbol
Nek1??[Similar Products]
NCBI Official Synonym Symbols
kat; D8Ertd790e
??[Similar Products]
NCBI Protein Information
serine/threonine-protein kinase Nek1
UniProt Protein Name
Serine/threonine-protein kinase Nek1
UniProt Synonym Protein Names
Never in mitosis A-related kinase 1; NimA-related protein kinase 1
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
Nek1??[Similar Products]
UniProt Synonym Gene Names
NimA-related protein kinase 1??[Similar Products]
UniProt Entry Name
NEK1_MOUSE
UniProt Comments for NEK1
NEK1: a protein kinase related to the NIMA (never in mitosis, gene A) serine/threonine kinase of Aspergillus nidulans. Expressed at high levels during gametogenesis and neurons of the peripheral ganglia. Implicated in the control of meiosis. Mouse mutants lead to facial malformation, male sterility and polycystic kidney disease. Two alternatively spliced human isoforms have been reported.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NEK family
Cellular Component: centrosome; pericentriolar material; cytoskeleton; cytoplasm; nucleus
Molecular Function: transferase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity
Biological Process: mitosis; activation of MAPK activity; multicellular organism growth; cell cycle; protein amino acid phosphorylation; cell projection organization and biogenesis; cell division; cilium biogenesis; spermatogenesis; response to ionizing radiation; kidney development; response to DNA damage stimulus; phosphorylation
Research Articles on NEK1
1. Nek1 is detectable in all murine tissues but its expression in wild type and kat2J heterozygous kidneys decrease as the kidneys mature. In the embryonic kidney, Nek1 expression is most prominent in cells that will become podocytes and proximal tubules.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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