ALK tyrosine kinase receptor; Anaplastic lymphoma kinase; CD246; ALK

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This ALK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-666 amino acids from the Central region of human ALK.

Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Vial Concentration: 2 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-ALK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

ALK, a member of the insulin receptor subfamily of Tyr protein kinases, is an orphan receptor. It appears to play an important role in the normal development and function of the nervous system. This Type I membrane protein is expressed in brain and CNS and in the small intestine and testis, but not in normal lymphoid cells. A form of non-Hodgkin's lymphoma is characterized by a chromosomal translocation t(2;5)(p23;q35) that involves NPM1 and ALK. The protein contains 1 LDL-receptor class A domain and 2 putative MAM domains.

Cancer; Neuroscience

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

WB~~1:1000

Western blot analysis of ALK(Center)(arrow) using rabbit polyclonal ALK(Center) Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALK (Center) gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human testis tissue reacted with ALK antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

176442

anaplastic lymphoma receptor tyrosine kinase

ALK tyrosine kinase receptor

ALK tyrosine kinase receptor

ALK_HUMAN

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors

Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; Oncoprotein; Protein kinase, tyrosine (receptor); TK group; Alk family

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: protein complex; integral to plasma membrane

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: regulation of apoptosis; cell proliferation; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; neuron development; signal transduction; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; activation of NF-kappaB transcription factor

Disease: Neuroblastoma, Susceptibility To, 3

Morris, S.W., et al., Oncogene 14(18):2175-2188 (1997).
Iwahara, T., et al., Oncogene 14(4):439-449 (1997).
Morris, S.W., et al., Science 263(5151):1281-1284 (1994).
Morris, S.W., et al., Oncogene 15, 2883-2883 (1997).

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