RP11-331H2.1; MGC12523; MGC75018; PRAP; SDR37C1; 17 beta-hydroxysteroid dehydrogenase type VII|17beta hydroxysteroid dehydrogenase|short chain dehydrogenase/reductase family 37C; member 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat HSD17B7. No significant cross-reactivity or interference between Rat HSD17B7 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of HSD17B7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284585 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 3-keto-steroid reductase (HSD17B7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSD17B7. The ELISA analytical biochemical technique of the MBS284585 kit is based on HSD17B7 antibody-HSD17B7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSD17B7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSD17B7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HSD17B7 in samples. An antibody specific for HSD17B7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHSD17B7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSD17B7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSD17B7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

37,372 Da

hydroxysteroid (17-beta) dehydrogenase 7

3-keto-steroid reductase

3-keto-steroid reductase

17-beta-HSD 7; PRAP??[Similar Products]

a phosphoprotein that associates with the short form of the prolactin receptor [RGD, Feb 2006]

HSD17B7: Responsible for the reduction of the keto group on the C-3 of sterols. Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.270; EC 1.1.1.62; Endoplasmic reticulum; Lipid Metabolism - androgen and estrogen; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 13q24

Cellular Component: endoplasmic reticulum; integral component of membrane; membrane; plasma membrane

Molecular Function: 3-keto sterol reductase activity; estradiol 17-beta-dehydrogenase activity; prolactin receptor binding

Biological Process: cholesterol biosynthetic process; estrogen biosynthetic process; hippocampus development; maternal process involved in female pregnancy; response to drug; response to estradiol; response to organic cyclic compound

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.