Full Product Name
CFH Polyclonal Antibody
Product Synonym Names
AHUS1; AMBP1; ARMD4; ARMS1; CFHL3; FH; FHL1; HF; HF1; HF2; HUS
Product Gene Name
anti-CFH antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
PYIPNGDYSP LRIKHRTGDE ITYQCRNGFY PATRGNTAKC TSTGWIPAPR CTLKPCDYPD IKHGGLYHEN MRRPYFPVAV GKYYSYYCDE HFETPSGSYW DHIHCTQDGW SPAVPCLRKC YFPYLENGYN QNHGRKFVQG KSIDVACHPG YALPKAQTTV TCMENGWSPT PRCIRV
3D Structure
ModBase 3D Structure for P08603
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human CFH
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Secreted
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-CFH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CFH antibody
This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Product Categories/Family for anti-CFH antibody
Polyclonal Antibodies
Applications Tested/Suitable for anti-CFH antibody
Western Blot (WB)
Application Notes for anti-CFH antibody
WB: 1:500 - 1:2000
Western Blot (WB) of anti-CFH antibody
Western blot analysis of extracts of HepG2 cells, using CFH antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
NCBI/Uniprot data below describe general gene information for CFH. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000177.2
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NCBI GenBank Nucleotide #
NM_000186.3
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UniProt Primary Accession #
P08603
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UniProt Secondary Accession #
P78435; Q14570; Q2TAZ5; Q38G77; Q5TFM3; Q8N708; Q9NU86; A5PL14[Other Products]
UniProt Related Accession #
P08603[Other Products]
Molecular Weight
Calculated: 51kDa; 139kDa
Observed: 150kDa
NCBI Official Full Name
complement factor H isoform a
NCBI Official Synonym Full Names
complement factor H
NCBI Official Symbol
CFH??[Similar Products]
NCBI Official Synonym Symbols
FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3
??[Similar Products]
NCBI Protein Information
complement factor H
UniProt Protein Name
Complement factor H
UniProt Synonym Protein Names
H factor 1
Protein Family
Complement factor
UniProt Gene Name
CFH??[Similar Products]
UniProt Synonym Gene Names
HF; HF1; HF2??[Similar Products]
NCBI Summary for CFH
This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011]
UniProt Comments for CFH
Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.
Research Articles on CFH
1. inhibition of the alternative pathway by factor H, with a concentration equivalent to a high physiological level, strongly reduced C5a levels and decreased proinflammatory cytokine production in human peripheral blood mononuclear cells.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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