Product Name
MTMR2, siRNA
Full Product Name
MTMR2 siRNA (Human)
Product Synonym Names
KIAA1073; Myotubularin-related protein 2
Product Gene Name
MTMR2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13614
Specificity
MTMR2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MTMR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MTMR2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MTMR2 sirna
siRNA to inhibit MTMR2 expression using RNA interference
Applications Tested/Suitable for MTMR2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MTMR2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001230500.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001243571.1
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UniProt Primary Accession #
Q13614
[Other Products]
UniProt Secondary Accession #
Q9UPS9; A6NN98[Other Products]
UniProt Related Accession #
Q13614[Other Products]
Molecular Weight
65,959 Da
NCBI Official Full Name
myotubularin-related protein 2 isoform 2
NCBI Official Synonym Full Names
myotubularin related protein 2
NCBI Official Symbol
MTMR2??[Similar Products]
NCBI Official Synonym Symbols
CMT4B; CMT4B1
??[Similar Products]
NCBI Protein Information
myotubularin-related protein 2
UniProt Protein Name
Myotubularin-related protein 2
UniProt Synonym Protein Names
Phosphatidylinositol-3,5-bisphosphate 3-phosphatase (EC:3.1.3.95
Protein Family
Myotubularin-related protein
UniProt Gene Name
MTMR2??[Similar Products]
UniProt Synonym Gene Names
KIAA1073??[Similar Products]
UniProt Entry Name
MTMR2_HUMAN
NCBI Summary for MTMR2
This gene is a member of the myotubularin family of phosphoinositide lipid phosphatases. The encoded protein possesses phosphatase activity towards phosphatidylinositol-3-phosphate and phosphatidylinositol-3,5-bisphosphate. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4B, an autosomal recessive demyelinating neuropathy. Alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniProt Comments for MTMR2
MTMR2: Phosphatase that acts on lipids with a phosphoinositol headgroup. Has phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. Homooligomer and heterooligomer. Interacts with SBF1 and SBF2. interaction with SBF1 increases phosphatase activity. Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.
Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - fructose and mannose; Phosphatase, lipid; Protein phosphatase, tyrosine (non-receptor); Cofactor and Vitamin Metabolism - riboflavin; Cofactor and Vitamin Metabolism - thiamine; EC 3.1.3.64; EC 3.1.3.95
Chromosomal Location of Human Ortholog: 11q22
Cellular Component: synaptic vesicle; axon; early endosome membrane; cytoplasm; dendrite; vacuolar membrane; dendritic spine; cytosol; nucleus
Molecular Function: protein binding; phosphatidylinositol-3-phosphatase activity; protein homodimerization activity; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity
Biological Process: myelin formation; negative regulation of myelination; negative regulation of endocytosis; inositol phosphate dephosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; neuron development; phosphoinositide dephosphorylation; protein amino acid dephosphorylation; protein tetramerization; negative regulation of receptor internalization
Disease: Charcot-marie-tooth Disease, Type 4b1
Research Articles on MTMR2
1. we identified a novel mutation in MTMR2 in a family with CMT4B1 and myelin outfoldings
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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