Product Name
Alpha-2C Adrenergic (ALPHA2CAR), Monoclonal Antibody
Full Product Name
Alpha-2C Adrenergic Antibody, Clone S330A-51: APC/Cy7
Product Synonym Names
Mouse Anti-Human Alpha 2C Adrenergic Monoclonal IgG2A; Alpha 2C Adrenergic Antibody: APC/Cy7; Adra 2c Antibody; Alpha-2CAR Antibody; ADRA2C Antibody; ADRA2L2 Antibody; ADRARL2 Antibody; Alpha 2CAR Antibody; alpha2 AR C4 Antibody; alpha2 C4 Antibody; alpha2C Antibody; ALPHA2CAR Antibody; Alpha-2 adrenergic receptor subtype C4 Antibody; Alpha-2C adrenoreceptor Antibody; Adrenergic alpha 2C receptor Antibody; Alpha 2C adrenergic receptor Antibody; Alpha 2C adrenergic receptor subtype C4 Antibody; Alpha 2C adrenoceptor Antibody; Alpha 2C adrenoreceptor Antibody; Alpha-2-adrenergic receptor Antibody; renal type Antibody; alpha2cii-adrenergic receptor Antibody; Subtype alpha2 C4 Antibody
Product Gene Name
anti-ALPHA2CAR antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P18825
Species Reactivity
Human, Mouse
Specificity
Detects ~50 kDa or larger (possibly due to dimerization). Does not cross-react with other adrenergic receptors.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide amino acids 442-462 (QDFRRSFKHILFRRRRRGFRQ, cytoplasmic C-terminus) of human Alpha-2C adrenergic receptor
Cellular Localization
Cell Membrane
Certificate of Analysis
2 ug/ml was sufficient for detection of Alpha-2C Adrenergic Receptor in 20 ug of COS cells transiently transfected with HA-tagged Alpha-2Cmembrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the seconbody.y.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, F?rster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-ALPHA2CAR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ALPHA2CAR antibody
The alpha 2C Adrenergic Receptor controls the release of neurotransmitter from central adrenergic neurons and from sympathetic nerves in the heart. This receptor also plays a role in cognitive and behavioral function. Two variants are produced by alternative splicing. Alpha 2C Adrenergic Receptor has been reported extensively in the brain. Blood, corpus cavermosum, kidney, and heart tissues have also been reported to express the protein.
Product Categories/Family for anti-ALPHA2CAR antibody
Neuroscience; Neurotransmitter Receptors; Alpha-2 Adrenergic Receptors; Cell Signaling
Applications Tested/Suitable for anti-ALPHA2CAR antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ALPHA2CAR antibody
WB: 1:1000
Immunocytochemistry (ICC) of anti-ALPHA2CAR antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha-2C Adrenergic Receptor Monoclonal Antibody, Clone S330A-51 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha-2C Adrenergic Receptor Antibody. (D) Composite.
Western Blot (WB) of anti-ALPHA2CAR antibody
Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa Alpha 2C Adrenergic
NCBI/Uniprot data below describe general gene information for ALPHA2CAR. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000674.2
[Other Products]
NCBI GenBank Nucleotide #
NP_000674.2
[Other Products]
UniProt Primary Accession #
P18825
[Other Products]
UniProt Related Accession #
P18825[Other Products]
NCBI Official Full Name
alpha-2C adrenergic receptor
NCBI Official Synonym Full Names
adrenoceptor alpha 2C
NCBI Official Symbol
ADRA2C??[Similar Products]
NCBI Official Synonym Symbols
ADRA2L2; ADRARL2; ADRA2RL2; ALPHA2CAR
??[Similar Products]
NCBI Protein Information
alpha-2C adrenergic receptor
UniProt Protein Name
Alpha-2C adrenergic receptor
UniProt Synonym Protein Names
Alpha-2 adrenergic receptor subtype C4; Alpha-2C adrenoreceptor
Protein Family
Alpha-2C adrenergic receptor
UniProt Gene Name
ADRA2C??[Similar Products]
UniProt Synonym Gene Names
ADRA2L2; ADRA2RL2; Alpha-2C adrenoceptor; Alpha-2CAR??[Similar Products]
UniProt Entry Name
ADA2C_HUMAN
NCBI Summary for ALPHA2CAR
Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]
UniProt Comments for ALPHA2CAR
ADRA2C: Alpha-2 adrenergic receptors mediate the catecholamine- induced inhibition of adenylate cyclase through the action of G proteins. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2C sub-subfamily.
Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4p16
Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; nerve terminal; endosome
Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; alpha2-adrenergic receptor activity; epinephrine binding; alpha-2A adrenergic receptor binding
Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; activation of protein kinase B; energy reserve metabolic process; positive regulation of vasoconstriction; negative regulation of epinephrine secretion; female pregnancy; regulation of sensory perception of pain; positive regulation of neuron differentiation; blood coagulation; regulation of insulin secretion
Product References and Citations for anti-ALPHA2CAR antibody
1. Filipeanu C.M., et al. (2011) Biochim BiophysActa. 1813: 346-357. 2. Powe D.G., et al. (2011) Breast Cancer Res Treat. 130: 457-463.
Research Articles on ALPHA2CAR
1. alpha2A- and alpha2C-adrenoceptors are functional receptors for norepinephrine, dopamine, and other previously assumed selective D2-like receptor ligands.
Precautions
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