Product Name
2',5'-Oligoadenylate Synthetase Like Protein (OASL), ELISA Kit
Full Product Name
Human 2',5'-Oligoadenylate Synthetase Like Protein (OASL) ELISA Kit
Product Synonym Names
TRIP14; p59OASL; 59 kDa 2'-5'-oligoadenylate synthase-like protein; Thyroid receptor-interacting protein 14
Product Gene Name
OASL elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
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3D Structure
ModBase 3D Structure for Q15646
Specificity
This assay has high sensitivity and excellent specificity for detection of 2',5'-Oligoadenylate Synthetase Like Protein (OASL).
No significant cross-reactivity or interference between 2',5'-Oligoadenylate Synthetase Like Protein (OASL) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Cell Lysates and Other Biological Fluids
Detection Range
15.62-1000pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level 2',5'-Oligoadenylate Synthetase Like Protein (OASL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level 2',5'-Oligoadenylate Synthetase Like Protein (OASL) were tested on 3 different plates, 8 replicates in each plate.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of OASL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for OASL purchase
MBS2705888 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 2',5'-Oligoadenylate Synthetase Like Protein (OASL) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OASL. The ELISA analytical biochemical technique of the MBS2705888 kit is based on OASL antibody-OASL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OASL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OASL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
OASL elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 2',5'-Oligoadenylate Synthetase Like Protein (OASL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 2',5'-Oligoadenylate Synthetase Like Protein (OASL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 2',5'-Oligoadenylate Synthetase Like Protein (OASL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 2',5'-Oligoadenylate Synthetase Like Protein (OASL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Typical Testing Data/Standard Curve (for reference only) of OASL elisa kit
NCBI/Uniprot data below describe general gene information for OASL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001248754.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001261825.1
[Other Products]
UniProt Primary Accession #
Q15646
[Other Products]
UniProt Secondary Accession #
O75686; Q17R95; Q9Y6K6; Q9Y6K7; B2RAZ2; I1YDD2[Other Products]
UniProt Related Accession #
Q15646[Other Products]
Molecular Weight
43,942 Da
NCBI Official Full Name
2'-5'-oligoadenylate synthase-like protein isoform c
NCBI Official Synonym Full Names
2'-5'-oligoadenylate synthetase like
NCBI Official Symbol
OASL??[Similar Products]
NCBI Official Synonym Symbols
OASL1; OASLd; TRIP14; TRIP-14; p59OASL; p59 OASL; p59-OASL
??[Similar Products]
NCBI Protein Information
2'-5'-oligoadenylate synthase-like protein
UniProt Protein Name
2'-5'-oligoadenylate synthase-like protein
UniProt Synonym Protein Names
2'-5'-OAS-related protein; 2'-5'-OAS-RP; 59 kDa 2'-5'-oligoadenylate synthase-like protein; Thyroid receptor-interacting protein 14; TR-interacting protein 14; TRIP-14; p59 OASL; p59OASL
Protein Family
2'-5'-oligoadenylate synthase-like protein
UniProt Gene Name
OASL??[Similar Products]
UniProt Synonym Gene Names
TRIP14; 2'-5'-OAS-RP; TR-interacting protein 14; TRIP-14; p59OASL??[Similar Products]
UniProt Comments for OASL
Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L.
Research Articles on OASL
1. These data uncover a promycobacterial role for STING-dependent OASL production during Mycobacterium leprae infection that directs the host immune response toward a niche that permits survival of the pathogen.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.