Full Product Name
Elastin, human
Product Gene Name
anti-ELN antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15502
Species Reactivity
Human, Rat (< 0.1%)
Purity/Purification
Affinity Purified
Form/Format
Lyophilized from phosphate buffered solution; no BSA and preservative added.
Concentration
1 mg/ml (lot specific)
Immunogen
Purified elastin from human skin
Positive Control
Human skin or aorta
Incubation Time
IHC(P) 60 min at RT or 2-8 degree C over night
Pre-Treatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-ELN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ELN antibody
Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties. Human elastin, cross-reactivity in RIA at 1:500 dilution: human collagen type I, II, III, IV, V <0.1 %; human fibronectin < 0.1%.
Applications Tested/Suitable for anti-ELN antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Radioimmunoassay (RIA)
Application Notes for anti-ELN antibody
IFA: 1:80
IHC-P: 1:500
ELISA: 1:200
NCBI/Uniprot data below describe general gene information for ELN. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000492.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000501.3
[Other Products]
UniProt Primary Accession #
P15502
[Other Products]
UniProt Secondary Accession #
O15336; O15337; Q14233; Q14234; Q14235; Q14238; Q6P0L4; Q6ZWJ6; Q75MU5; Q7Z316; B3KTS6[Other Products]
UniProt Related Accession #
P15502[Other Products]
Molecular Weight
60,980 Da[Similar Products]
NCBI Official Full Name
elastin isoform a
NCBI Official Synonym Full Names
elastin
NCBI Official Symbol
ELN??[Similar Products]
NCBI Official Synonym Symbols
WS; WBS; SVAS; ADCL1
??[Similar Products]
NCBI Protein Information
elastin
UniProt Protein Name
Elastin
UniProt Synonym Protein Names
Tropoelastin
UniProt Gene Name
ELN??[Similar Products]
NCBI Summary for ELN
This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. [provided by RefSeq, Aug 2017]
UniProt Comments for ELN
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle ().
Product References and Citations for anti-ELN antibody
1. Black A.F., Bouez C., Perrier E., Schlotmann K., Chapuis F., Damour O. (2005) Optimization and characterization of an Engineered human skin equivalent. Tissue Engineering 11, 723-733. 2. Guerret S., Govignon E., Hartmann D.J., Ronfard V. (2003) Long term remodeling of a bilayered living human skin equivalent (Apligraf) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 3. Zsolt U., Peyrol S., Plauchu H., Zabot M.T., Lebwohl M., Schilling K., Green M., Boyd C.D., Csiszár K. (2000) Elastin gene deletions in Williams syndrome patients result in altered deposition of elastic fibers in skin and a subclinical dermal phenotype. 4. Duplan-Perrat F., Damour O., Montrocher C., Peyrol S., Grenier G., Jacob M.P., Braye F. (2000) Keratinocytes influence the maturation and organization of the elastin network in a skin equivalent. J. Invest Dermatol 114, 365-370.
Research Articles on ELN
1. exogenous growth factors enhance the expression of cola1, cola3, and Elastin, which is probably regulated via activating MAPK signaling pathway.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
