Human Forkhead box protein N1 (FOXN1) ELISA kit; FKHL20; RONU; WHN; Rowett nude; winged helix nude; winged-helix nude; forkhead box N1

For Research Use Only. Not for use in diagnostic procedures.

No significant cross-reactivity or interference between Human FOXN1 and analogues was observed.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of FOXN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9330404 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the forkhead box N1 (FOXN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FOXN1. The ELISA analytical biochemical technique of the MBS9330404 kit is based on FOXN1 antibody-FOXN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FOXN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FOXN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FOXN1 concentrations in Human serum, plasma and other body fluids. Using Purified Human FOXN1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FOXN1 and FOXN1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FOXN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

68,925 Da

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Forkhead box protein N1

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FOXN1_HUMAN

Mutations in the winged-helix transcription factor gene at the nude locus in mice and rats produce the pleiotropic phenotype of hairlessness and athymia, resulting in a severely compromised immune system. This gene is orthologous to the mouse and rat genes and encodes a similar DNA-binding transcription factor that is thought to regulate keratin gene expression. A mutation in this gene has been correlated with T-cell immunodeficiency, the skin disorder congenital alopecia, and nail dystrophy. Alternative splicing in the 5' UTR of this gene has been observed. [provided by RefSeq, Jul 2008]

FOXN1: Transcriptional regulator involved in development. Defects in FOXN1 are the cause of T-cell immunodeficiency congenital alopecia and nail dystrophy (TIDAND). A disorder characterized by the association of congenital alopecia, severe T-cell immunodeficiency, and ridging and pitting of all nails.

Protein type: Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; keratinocyte differentiation; epidermis development; organ morphogenesis; epithelial cell proliferation; hair follicle development; thymus development; regulation of T cell differentiation in the thymus; defense response; lymphocyte homeostasis

Disease: T-cell Immunodeficiency, Congenital Alopecia, And Nail Dystrophy

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