Full Product Name
Anti-DAP12 Antibody (aa85-113) IHC-plus
Product Synonym Names
Rabbit Polyclonal to Human DAP12; Human DAP12; DNAX-activation protein 12; PLOSL; KARAP; KAR-associated protein; PLO-SL
Product Gene Name
anti-DAP12 antibody
[Similar Products]
Product Synonym Gene Name
TYROBP[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O43914
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, 0.09% sodium azide
Immunogen
DAP12 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human TYROBP.
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human TYROBP.
Immunogen Type
Synthetic peptide
Antigen Modification
aa85-113
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
Other Notes
Small volumes of anti-DAP12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DAP12 antibody
TYROBP Antibody, DNAX-activation protein 12 Antibody, DAP12 Antibody, PLOSL Antibody, KARAP Antibody, KAR-associated protein Antibody, PLO-SL Antibody Description: DAP12 is a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element.
Applications Tested/Suitable for anti-DAP12 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-DAP12 antibody
Flow Cytometry (1:10 - 1:50)
IHC-P (1:100)
WB (1:1000)
Immunohistochemistry (IHC) of anti-DAP12 antibody
Anti-DAP12 antibody IHC staining of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western Blot (WB) of anti-DAP12 antibody
Western blot of TYROBP Antibody in HepG2 cell line lysates (35 ug/lane). TYROBP (arrow) was detected using the purified antibody.

Flow Cytometry (FC/FACS) of anti-DAP12 antibody
TYROBP Antibody flow cytometry of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

NCBI/Uniprot data below describe general gene information for DAP12. It may not necessarily be applicable to this product.
NCBI Accession #
NP_003323.1
[Other Products]
NCBI GenBank Nucleotide #
NM_003332.3
[Other Products]
UniProt Primary Accession #
O43914
[Other Products]
UniProt Secondary Accession #
Q6FGA5; Q9UMT3; A8K2X0; F5H389[Other Products]
UniProt Related Accession #
O43914[Other Products]
Molecular Weight
11,043 Da
NCBI Official Full Name
TYRO protein tyrosine kinase-binding protein isoform 1
NCBI Official Synonym Full Names
TYRO protein tyrosine kinase binding protein
NCBI Official Symbol
TYROBP??[Similar Products]
NCBI Official Synonym Symbols
DAP12; KARAP; PLOSL
??[Similar Products]
NCBI Protein Information
TYRO protein tyrosine kinase-binding protein
UniProt Protein Name
TYRO protein tyrosine kinase-binding protein
UniProt Synonym Protein Names
DNAX-activation protein 12; Killer-activating receptor-associated protein; KAR-associated protein
UniProt Gene Name
TYROBP??[Similar Products]
UniProt Synonym Gene Names
DAP12; KARAP; KAR-associated protein??[Similar Products]
UniProt Entry Name
TYOBP_HUMAN
NCBI Summary for DAP12
This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Mar 2010]
UniProt Comments for DAP12
TYROBP: Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Belongs to the TYROBP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: cell surface; integral to plasma membrane; intracellular; plasma membrane
Molecular Function: identical protein binding; protein binding; receptor binding; receptor signaling protein activity
Biological Process: axon guidance; cellular defense response; innate immune response; integrin-mediated signaling pathway; macrophage activation during immune response; neutrophil activation during immune response; regulation of immune response; signal transduction
Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy
Research Articles on DAP12
1. This study reveled that TYROBP having a central role in the bipolar disease and schizophrenia manifestation.
Precautions
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