Product Name
Interleukin-1 (IL1RAPL1), ELISA Kit
Full Product Name
Human Interleukin-1 receptor accessory protein-like 1 (IL1RAPL1) ELISA Kit
Product Synonym Names
IL1R8; IL1RAPL; MRX10; MRX21; MRX34; OPHN4; TIGIRR-2; OTTHUMP00000023097|X-linked interleukin-1 receptor accessory protein-like 1|interleukin 1 receptor-8|mental retardation; X-linked 10|oligophreni
Product Gene Name
IL1RAPL1 elisa kit
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for Q9NZN1
Specificity
This assay has high sensitivity and excellent specificity for detection of Human IL1RAPL1. No significant cross-reactivity or interference between Human IL1RAPL1 and analogues was observed.
Samples
Serum, Plasma, Other
biological fluids
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of IL1RAPL1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IL1RAPL1 purchase
MBS283828 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-1 (IL1RAPL1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL1RAPL1. The ELISA analytical biochemical technique of the MBS283828 kit is based on IL1RAPL1 antibody-IL1RAPL1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL1RAPL1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL1RAPL1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
IL1RAPL1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL1RAPL1 in samples. An antibody specific for IL1RAPL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL1RAPL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1RAPL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1RAPL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI/Uniprot data below describe general gene information for IL1RAPL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055086.1
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NCBI GenBank Nucleotide #
NM_014271.3
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UniProt Primary Accession #
Q9NZN1
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UniProt Secondary Accession #
Q9UJ53; A0AVG4[Other Products]
UniProt Related Accession #
Q9NZN1[Other Products]
Molecular Weight
79,969 Da
NCBI Official Full Name
interleukin-1 receptor accessory protein-like 1
NCBI Official Synonym Full Names
interleukin 1 receptor accessory protein like 1
NCBI Official Symbol
IL1RAPL1??[Similar Products]
NCBI Official Synonym Symbols
IL1R8; MRX10; MRX21; MRX34; OPHN4; IL1RAPL; TIGIRR-2; IL1RAPL-1; IL-1RAPL-1; IL-1-RAPL-1
??[Similar Products]
NCBI Protein Information
interleukin-1 receptor accessory protein-like 1
UniProt Protein Name
Interleukin-1 receptor accessory protein-like 1
UniProt Synonym Protein Names
Oligophrenin-4; Three immunoglobulin domain-containing IL-1 receptor-related 2; TIGIRR-2; X-linked interleukin-1 receptor accessory protein-like 1
Protein Family
Interleukin
UniProt Gene Name
IL1RAPL1??[Similar Products]
UniProt Synonym Gene Names
OPHN4; IL-1-RAPL-1; IL-1RAPL-1; IL1RAPL-1; TIGIRR-2??[Similar Products]
NCBI Summary for IL1RAPL1
The protein encoded by this gene is a member of the interleukin 1 receptor family and is similar to the interleukin 1 accessory proteins. This protein has an N-terminal signal peptide, three extracellular immunoglobulin Ig-like domains, a transmembrane domain, an intracellular Toll/IL-1R domain, and a long C-terminal tail which interacts with multiple signalling molecules. This gene is located at a region on chromosome X that is associated with a non-syndromic form of X-linked intellectual disability. Deletions and mutations in this gene were found in patients with intellectual disability. This gene is expressed at a high level in post-natal brain structures involved in the hippocampal memory system, which suggests a specialized role in the physiological processes underlying memory and learning abilities, and plays a role in synapse formation and stabilization. [provided by RefSeq, Jul 2017]
UniProt Comments for IL1RAPL1
IL1RAPL1: May regulate secretion and presynaptic differentiation through inhibition of the activity of N-type voltage-gated calcium channel. May activate the MAP kinase JNK. Plays a role in presynaptic and postsynaptic differentiation and dendritic spine formation in neurons. Defects in IL1RAPL1 are the cause of mental retardation X-linked type 21 (MRX21). Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non- syndromic mental retardation patients do not manifest other clinical signs. Belongs to the interleukin-1 receptor family. 1 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: Xp21.3-p21.2
Cellular Component: axon; cell surface; cytoplasm; dendrite; integral component of membrane; plasma membrane; postsynaptic membrane
Molecular Function: interleukin-1 binding; protein binding; receptor binding; voltage-gated calcium channel activity
Biological Process: heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; negative regulation of exocytosis; neuron differentiation; positive regulation of dendrite morphogenesis; positive regulation of synaptogenesis; signal transduction
Disease: Mental Retardation, X-linked 21
Research Articles on IL1RAPL1
1. Altered DNA methylation in IL1RAPL1 involves in the etiology of Bipolar disorder and Major Depressive disorder .
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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