Full Product Name
Elastin, rat
Product Gene Name
anti-Eln antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q99372
Species Reactivity
Rat, Mouse elastin (strong), Human elastin (<0,1%)
Purity/Purification
Affinity Purified
Form/Format
Lyophilized from phosphate buffered solution; no BSA and preservative added.
Concentration
1 mg/ml (lot specific)
Immunogen
Purified elastin from rat aorta
Positive Control
Rat skin or aorta
Incubation Time
IHC(P) 60 min at RT or 2-8 degree C over night
Pre-Treatment
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 degree C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Preparation and Storage
Store at -20 degree C.
Other Notes
Small volumes of anti-Eln antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Eln antibody
Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties. Rat elastin 100%, rat collagen type I, II, III and V <0.1% (RIA at 1:500 dilution).
Applications Tested/Suitable for anti-Eln antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Radioimmunoassay (RIA)
Application Notes for anti-Eln antibody
IF: 1:40
IHC-P: 1:500
ELISA: 1:200
NCBI/Uniprot data below describe general gene information for Eln. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036854.1
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NCBI GenBank Nucleotide #
NM_012722.1
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UniProt Primary Accession #
Q99372
[Other Products]
UniProt Secondary Accession #
Q5RKH4[Other Products]
UniProt Related Accession #
Q99372[Other Products]
Molecular Weight
67,604 Da[Similar Products]
NCBI Official Full Name
elastin
NCBI Official Synonym Full Names
elastin
NCBI Official Symbol
Eln??[Similar Products]
NCBI Official Synonym Symbols
Trela; TREL11; Trela26; RATTREL11
??[Similar Products]
NCBI Protein Information
elastin
UniProt Protein Name
Elastin
UniProt Synonym Protein Names
Tropoelastin
UniProt Gene Name
Eln??[Similar Products]
NCBI Summary for Eln
precursor of elastin, which is a major component of the elastic fibers of the extracellular matrix; confers elasticity to tissues [RGD, Feb 2006]
UniProt Comments for Eln
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle ().
Product References and Citations for anti-Eln antibody
1. Desmoulière A., Darby I., Monte Alto Costa A., Raccurt M., Tuchweber B., Sommer P., Gabbiani G. (1997) Extracellular Matrix Deposition, lysyl oxidase expression, and myofibroblastic differentiation during the initial stages of cholestatic fibrosis in the rat. Lab Invest.76(6):765-78. 2. Lethias C., Chignier E., Garrone R., Hartmann D.J., Eloy R. (1988) Elastogenesis in rat arterial grafts: elastin deposits on microfibrillar and non-microfibrillar structures. Histochem. J. 20, 715-721. 3. Ruggiero F., Lethias C., Garrone R. (1987) Immunolabelling of freeze-fractured tissue: thin sections of rat aorta after labelling of elestic laminae. Eur. J. Cell. Biol. 44, 90-92. 4. Lethias C., Hartmann D.J., Masmejean M., Ravazzola M., Sabbagh I., Ville G., Herbage D., Eloy R. (1987) Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique. J. Histochem. Cytochem. 35, 15-21.
Research Articles on Eln
1. Overall, this study suggested that UC-MSCs could ameliorate aberrant elastin expression in the lung of hyperoxia-induced BPD model which may be associated with suppressing increased TGFbeta1 activation.
Precautions
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