Full Product Name
A1CF antibody - N-terminal region
Product Gene Name
anti-A1CF antibody
[Similar Products]
Product Synonym Gene Name
ACF; ACF64; ACF65; APOBEC1CF; ASP; MGC163391; RP11-564C4.2[Similar Products]
Antibody/Peptide Pairs
A1CF peptide (MBS3230433) is used for blocking the activity of A1CF antibody (MBS3205468)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: MESNHKSGDG LSGTQKEAAL RALVQRTGYS LVQENGQRKY GGPPPGWDAA
3D Structure
ModBase 3D Structure for Q9NQ94
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human A1CF
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-A1CF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-A1CF antibody
This is a rabbit polyclonal antibody against A1CF. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. A1CF has three non-identica
Product Categories/Family for anti-A1CF antibody
Polyclonal; RNA Binding Proteins;
Applications Tested/Suitable for anti-A1CF antibody
Western Blot (WB)
Western Blot (WB) of anti-A1CF antibody
WB Suggested Anti-A1CF Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Spleen
NCBI/Uniprot data below describe general gene information for A1CF. It may not necessarily be applicable to this product.
NCBI Accession #
NP_055391
[Other Products]
NCBI GenBank Nucleotide #
NM_014576
[Other Products]
UniProt Primary Accession #
Q9NQ94
[Other Products]
UniProt Related Accession #
Q9NQ94[Other Products]
NCBI Official Full Name
A1CF protein
NCBI Official Synonym Full Names
APOBEC1 complementation factor
NCBI Official Symbol
A1CF??[Similar Products]
NCBI Official Synonym Symbols
ACF; ASP; ACF64; ACF65; APOBEC1CF
??[Similar Products]
NCBI Protein Information
APOBEC1 complementation factor
UniProt Protein Name
APOBEC1 complementation factor
UniProt Synonym Protein Names
APOBEC1-stimulating protein
Protein Family
APOBEC1 complementation factor
UniProt Gene Name
A1CF??[Similar Products]
UniProt Synonym Gene Names
ACF; ASP??[Similar Products]
UniProt Entry Name
A1CF_HUMAN
NCBI Summary for A1CF
Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Comments for A1CF
ACF: Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA processing; RNA-binding; Hydrolase
Chromosomal Location of Human Ortholog: 10q11.23
Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; apolipoprotein B mRNA editing enzyme complex
Molecular Function: protein binding; single-stranded RNA binding; RNA binding; double-stranded RNA binding; nucleotide binding
Biological Process: protein stabilization; cytidine to uridine editing; mRNA modification; gene expression; mRNA processing
Research Articles on A1CF
1. Interaction of any alcohol exposure with GCKR (rs780094) and A1CF (rs10821905) influenced the risk of gout in Europeans
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
